Morphology of the articular cartilage of the knee joint in rats with extraarticular femoral bone deformity

Konstantin Romanenko, Nataliya Ashukina, Inna Batura, Dmytro Prozorovsky


Objective: to assess the structural and functional state of the rat knee after modeling posttraumatic extraarticular deformation of the femur.

Methods: experiment was performed in 39 white mature (6 months old) male rats. To model the deformation of varus (test group, 15 rats) after transverse osteotomy of the fe­mur, a bent angled rod was introduced into the medullary ca­nal, the control animal (15) was straight. The comparison group was intact rats (9) of the corresponding age and sex. After 1, 3 and 6 months. After the operation, histological examination was performed.

Results: In 1 month after modeling the deformation in the articular cartilage of the knee joint, destructive changes of 1–2 degree according to OARSI were noted. In contralateral extremity, AC had a structure characteristic of the age norm. After 3 months a decrease in the width of articular cartilage (in comparison with intact group by 1.21 times, p < 0.01), loss of glycosaminoglycans, a violation of the structure and ordering of collagen fibers, accumulation of type I collagen was estab­lished. After 6 months the most pronounced disorders were found in the cerebral cortex on the medial condyle of the deformed femur — a 1.63 fold decrease in its width compared with the in­tact group (p < 0.001), in places the replacement with fibrous cartilage.

Conclusions: in animals with simulated extraarticular deformation of the femur in the knee joint of the injured limb after 1 month revealed destructive changes that progressed with time. After 1 month the maximum manifestation of disorders was noted in the area of the femur surface under the patella, and after 3 and 6 months he medial condyle. In the articular cartilage of the knee joint of the contralateral limb, destruc­tive changes were recorded 3 months after the reproduction of the deformation, but the severity of the manifestations was less.


posttraumatic deformation of the femur; knee joint; rats; osteoarthrosis; histological changes


Fayaz HC, Giannoudis PV, Vrahas MS, Smith RM, Moran C, Pape HC, Krettek C, Jupiter JB. The role stem cells in fracture healing and nonunion. Int Orthop. 2011;35(11):1586-97. doi: 10.1007/s00264-011-1338-z.

Popsuishapka O, Uzhigova O, Litvishko V. Rate of nonunion and delayed union of fragments in isolated diaphyseal fractures of long bones of the extremities. Orthopaedics, Traumatology and Prosthetics. 2013;(1):39–43. doi: 10.15674/0030-59872013139-43. (in Russian)

Engsberg J, Leduc S, Ricci W, Borrelli J Jr. Improved function and joint kinematics after correction of tibial malalignment. Am J Orthop (Belle Mead NJ). 2014 Dec;43(12):E313-8.

Marti RK, van Heerwaarden RJ. Osteotomies for posttraumatic deformities. First ed., Georg Thieme Verlag, 2008. 704 p.

Korzh M, Romanenko K, Karpinsky M, Prozorovsky D, Yaresko O. Mathematic modeling of the influence of femur malalignment on the bearing of lower extremity joints. Orthopaedics, Traumatology and Prosthetics. 2015;(4):25–30. doi: 10.15674/0030-59872015425-30. (in Ukrainian)

Bader DL, Salter DM, Chowdhury TT. Biomechanical influence of cartilage homeostasis in health and disease. Arthritis. 2011;2011:979032. doi: 10.1155/2011/979032.

Buckwalter JA, Martin JA, Brown TD. Perspectives on chondrocyte mechanobiology and osteoarthritis. Biorheology. 2006;43:603–9.

Fan CH. One-stage femoral osteotomy and computer-assisted navigation total knee arthroplasty for osteoarthritis in a patient with femoral subtrochanteric fracture malunion. Case Rep Orthop. 2014; 2014: 645927. doi: 10.1155/2014/645927.

Lonner JH, Siliski JM, Lotke PA. Simultaneous femoral osteotomy and total knee arthroplasty for treatment of osteoarthritis associated with severe extra-articular deformity. J Bone Joint Surg Am. 2000;82(3):342-8.

Deschamps G, Khiami F, Catonné Y, Chol C, Bussière C, Massin P; French Hip and Knee Society (S.F.H.G.). Total knee arthroplasty for osteoarthritis secondary to extra-articular malunions. Orthop Traumatol Surg Res. 2010 Dec;96(8):849-55. doi: 10.1016/j.otsr.2010.06.010.

Xiao-Gang Z, Shahzad K, Li C. One-stage total knee arthroplasty for patients with osteoarthritis of the knee and extra-articular deformity. Int Orthop. 2012; 36(12):2457–63. doi: 10.1007/s00264-012-1695-2.

European convention for the protection of vertebrate animals used for experimental and other scientific purposes. Council of Europe. Strasbourg, 18 Mar 1986.

Korzh M, Romanenko K, Ashukina N, Goridova L, Prozorovsky D. Methods for modelling femoral diaphysial deformities in rats. Orthopaedics, Traumatology and Prosthetics. 2010;(1):76–9. doi: 10.15674/0030-59872010176-79. (in Ukrainian)

Tyazhelov O, Poletaeva N, Romanenko K, Goridova L, Prozorovsky D. Mathematical modelling of diaphyseal deformities of the long bones. Orthopaedics, Traumatology and Prosthetics. 2010;(3):61–3. doi: 10.15674/0030-59872010361-63. (in Ukrainian)

Sarkisov DS, Perov YuL. Microscopic technics – Microskopicheskaya teknika. Moscow: Medicine, 1996. 542 p.

Kern M, Modish L, Dedukh NV, Malyshkina SV, Pankov EIa. [Principles of polarization-optical analysis in the study of connective tissue]. Arkh Anat Gistol Embriol. 1985 Jun;88(6):5-12. (in Russian)

Pritzker KP, Gay S, Jimenez SA, Ostergaard K, Pelletier JP, Revell PA, Salter D, van den Berg WB. Osteoarthritis cartilage histopathology: grading and staging. Osteoarthritis Cartilage. 2006;14:13–29. doi: 10.1016/j.joca.2005.07.014.

Gerwin N, Bendele AM, Glasson S, Carlson CS. The OARSI histopathology initiative – recommendations for histological assessments of osteoarthritis in the rat. Osteoarthritis Cartilage. 2010. Suppl 3:S24-34. doi: 10.1016/j.joca.2010.05.030.

Nam J, Aguda BD, Rath B, Agarwal S. Biomechanical thresholds regulate inflammation through the NF-kappaB pathway: experiments and modeling. PLoS One. 2009;4(4):e5262. doi: 10.1371/journal.pone.0005262.

Wei L, Hjerpe A, Brismar BH, Svensson O. Effect of load on articular cartilage matrix and the development of guinea-pig osteoarthritis. Osteoarthritis Cartilage. 2001 Jul;9(5):447-53.

Zhou Q, Wei B, Liu S, Mao F, Zhang X, Hu J, Zhou J, Yao Q, Xu Y, Wang L. Cartilage matrix changes in contralateral mobile knees in a rabbit model of osteoarthritis induced by immobilization. BMC Musculoskeletal Disorders. 2015 Aug 25;16:224. doi 10.1186/s12891-015-0679-y.

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