Regeneration and mechanical strength of bone in the implantation conditions of carbon material
DOI:
https://doi.org/10.15674/0030-59872016341-47Keywords:
carbon, bone regeneration, histological and biomechanical studiesAbstract
Objective: to study the regeneration of bone in the area of implantation of synthetic and felt-like dense carbon biomaterials and assess the strength of the femur quality with implanted biomaterials.
Methods: experiments performed in 36 white rats. Thick felt-like carbon and synthetic viscose biomaterial «Karbopon-22» implanted into transcortical perforated defect (3 × 3 mm) in metaphysis of the distal femur in rats. In control animals bone defect was not filled. Rats were euthanized on 14 and 45-th day. A histological study of material using topoptic techniques and biomechanical tests to evaluate axial load strength properties operated and contralateral femur has been performed.
Results: it is revealed bone formation around the implanted material, which increased the area of the observation period. Mineralized bone matrix based on collagen I type around the dense carbon formed. In the case of felt-like carbon spongy bone formed after 14 days, bone trabecular which are collagen type I, and after 45 days its fragments were buried in the fledgling bone with no signs of bone destruction. There is no evidence of inflammation, toxic effect in the surrounding bone and violation of reparative osteogenesis after the implantation of carbon material into «critical» size bone defect. It is determined that the load results in destruction of the bone after dense carbon material implantation by more than 1.8 times compared to the control and by 1.6 times compared with felt-like carbon implantation.
Conclusions: felt-like carbon can be recommended to fill «critical» bone defects in unloaded areas and dense in loaded and unloaded areas.References
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Copyright (c) 2016 Ninel Dedukh, Mykhaylo Karpinsky, Lu Chzhou, Svitlana Malyshkina
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