Regeneration and mechanical strength of bone in the implantation conditions of carbon material
Keywords:carbon, bone regeneration, histological and biomechanical studies
Objective: to study the regeneration of bone in the area of implantation of synthetic and felt-like dense carbon biomaterials and assess the strength of the femur quality with implanted biomaterials.
Methods: experiments performed in 36 white rats. Thick felt-like carbon and synthetic viscose biomaterial «Karbopon-22» implanted into transcortical perforated defect (3 × 3 mm) in metaphysis of the distal femur in rats. In control animals bone defect was not filled. Rats were euthanized on 14 and 45-th day. A histological study of material using topoptic techniques and biomechanical tests to evaluate axial load strength properties operated and contralateral femur has been performed.
Results: it is revealed bone formation around the implanted material, which increased the area of the observation period. Mineralized bone matrix based on collagen I type around the dense carbon formed. In the case of felt-like carbon spongy bone formed after 14 days, bone trabecular which are collagen type I, and after 45 days its fragments were buried in the fledgling bone with no signs of bone destruction. There is no evidence of inflammation, toxic effect in the surrounding bone and violation of reparative osteogenesis after the implantation of carbon material into «critical» size bone defect. It is determined that the load results in destruction of the bone after dense carbon material implantation by more than 1.8 times compared to the control and by 1.6 times compared with felt-like carbon implantation.Conclusions: felt-like carbon can be recommended to fill «critical» bone defects in unloaded areas and dense in loaded and unloaded areas.
Grigoryan A, Toporkova A. Problems of implants integrate into the bone tissue (theoretical aspects). Moskow: Technosphere, 2007. 128 p.
Korzh NA, Kladchenko LA, Malyshkina SV. Implantation materials and osteogenesis. The role of optimization and stimulation of bone reconstruction. Orthopedics, Traumatology and Prosthetics. 2008;(4):14–5.
Shevchenko SD, Movie AV. Substitution parietal diaphyseal bone defects of carbon implants. Orthopedics, Traumatology and Prosthetics. 1987;(7):39–8.
Hak DJ, Mauffrey C, Seligson D, Lind¬eque B. Use of carbon-fiber-reinforced composite implants in ortho¬pedic surgery. Orthopedics. 2014;37(12):825–830. doi: 10.3928/01477447-20141124-05.
Baker D, Kadambande SS, Alderman PM. Carbon fibre plates in the treatment of femoral peri-prosthetic fractures. Injury Int. J Care Injured. 2004;35:596–598.
Tzolkin PI, Cavalier GM, Sereda AP, et al. Carbon hip implant. Bulletin of the Russian Academy of Natural Sciences. 2015;(1):74–0.
Grabarczyk J, Ba¬tory D, Louda P, et al. Carbon coatings for medical implants. J Achievements Materials Manufactur¬ing Engineering. 2007;20(1–2):107–110.
Steinberg EL, Rath E, Shlaifer A, Chechik O, Maman E, Salai M. Carbon fiber reinforced PEEK Optima-a composite material biomechanical properties and wear/debris characteristics of CF-PEEK composites for orthopedic trauma implants. J Mech. Behav. Biomed. Mater. 2013;17:221-8. doi: 10.1016/j.jmbbm.2012.09.013.
Hillock R, Howard S. Utility of carbon fiber implants in orthopedic surgery: literature review. Re¬constructive Review. 2014;4(1):23–33. doi: 10.15438/rr.v4i1.55.
Zimel MN, Hwang S, Riedel ER, Healey JH. Carbon fiber intramedullary nails reduce artifact in postopera¬tive advanced imaging. Skeletal Radiology. 2015;44(9):1317–1325. doi: 10.1007/s00256-015-2158-9.
Rosenthal HG. Invisible stabilization of impending and pathological fractures a preliminary report on carbon fiber technology [web source]. Presented at the MSTS meeting. — 2013. — Accedd mode: http://media.wix.com/ugd/cd37de_2d070afa66c945689ebcb528a6cfbafd.pdf
European Convention for the Protection of vertebrate animals used for experimental and other scientific purposes. Strasbourg, 18 March 1986: official translation. Verkhovna Rada of Ukraine: http://zakon.rada.gov.ua/cgi–bin/laws/main.cginreg=994_137.
Law of Ukraine № 3447–IV of 21.02.2006 On protection of animals from cruelty. Article 26.
Sarkisov DS, Perov YuL. Microscopic technique. Moskow: Medicine, 1996. 542 p.
Schmitz N, Laverty S, Kraus VB, Aigner T. Basic methods in histopathology of joint tissues. Osteoarthritis Carti¬lage. 2010;18(3):113–116. doi: 10.1016/j.joca.2010.05.026.
Byuyul A, Tsefel P. SPSS. Art of information processing. Analysis of statistical data and restore hidden patterns. Trans. with it. Saint-Petersburg: LLC DiaSof», 2005. 608 p.
Tyazhelov OA, Tarasenko VI, Gurin IV. et al. Experimental and theoretical study of new technologies of osteosynthesis and replacement implants based on carbon of bone defects. Orthopedics, Traumatology and Prosthetics. 2008;4:46–1.
Tyazhelov AA, Komarov NP, Chertonkova EV, Poletaeva NY. Problems of replacement of bone defects and the role of carbon-carbon implants in their solution. Orthopedics, Traumatology and Prosthetics. 2008;4:128–3.
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