The effectiveness of the different schemes of complex conservative treatment of animals with induced knee arthritis
The effectiveness of conservative treatment in patients with knee joint arthritis can be enhanced through the use of the linked pharmacological and physical factors. The possibility of comprehensive use of low-intensity laser radiation (LILR) chondroprotectors and nonsteroidal anti-inflammatory drugs in patients with early knee joint arthritis- depending on the consistency of their application is not well studied. Objective: to study in experimental animals with induced knee joint arthritis effectiveness of different integrated schemes of conservative treatment.
Methods: we conducted experimental studyin 40 white rats aged 6 months. Knee joint arthritis stage I modeled by triple dexamethasone injections (7 mg/kg/m)performed once a week. The rats were divided into six groups depending on the treatment, which included 10 daily sessions of at la-ser therapy (a dose of 0.3 J contact on the knee joint) and per os administration of diclofenac sodium (8 mg/kg) and glucosamine hydrochloride (50 mg/kg) in the different sequences at intervals of 15 minutes. Microscopic examination of articular cartilage and biochemical blood serum analyses (glycoproteins, sialic acid, hondroyitynsulfat-, alkaline phosphatase) were carried out 28 days after the last injection of dexamethasone.
Results: in all rats in the experimental groups structural irregularities in articular cartilage were insignificant compared with the control. In animals after application LILRinitially and subsequent pharmacotherapy, morphological indicatorsof chondrocytes biosynthetic activity were most pronounced, and levels of serum inflammatory markers and alkaline phosphatase activity -were significantly lower than in the control and other research groups.
Conclusion: complex application of LILR with subsequent administration of diclofenac sodium glucosamine hydrochloride results in the most complete recovery of quantitative and structural-metabolic state of chondrocytes of articular cartilage and helps to normalize metabolic biochemical markers of cartilage and bone tissue.
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Belotskiy SM, Avtalion RR. Inflammation. Mobilization of cells and clinical effects. Moscow: Binom, 2008. 240 p.
Morozenko DV, Levchenko VI, Tymoshenko OP. Biochemical indicators of connective tissue in the diagnosis of diseases of dogs and cats: Guidelines. Bila Tserkva, 2012. 42 p.
Burianov ОА. Osteoarthritis: questions of pathogenesis, diagnosis and treatment. Zdorovia Ukrainy. 2009;23:30–2.
Vasilyeva LV, Starodubtseva IA. Pathogenetic rationale for the use of low-intensity laser radiation in patients with osteoarthritis. Journal of theoretical and practical medicine. Voronezh, 2007;5(1):13–7.
Kartashov МІ, Tymoshenko OP, Kibkalo DV. Veterinary Clinical Biochemistry: A Handbook. Kharkiv: Espada, 2010. 400 с.
Zupanets IA, Makolinets VI, Brun LV, Grashchenkova TN, Makolinets KV. Effect of sequence of application of low intensity laser radiation and the use of medicinal drugs in the treatment of osteoarthritis (experimentally-clinical research). In: Proceedings of the conference Strategy and Tactics of sanatorium rehabilitation of patients after treatment onkopatolohiy. The role of natural factors in medical sanatorium rehabilitation; 2010 October 4-5, Myrgorod:137.
Zupanets IA, Makolinets VI, Brun LV, Hrashchenkova TM, Diedukh NV, Malyshkina SV, Makolinets KV. Effect of integrated application of low infrared laser radiation and medications on the course of osteoarthritis (experimental research). In: Materials X Congress of the Republican health resort and physiotherapists. Current issues of the organization of the spa business, spa and physiotherapy Policy of the Autonomous Republic of Crimea; 2010 April 13-14, Yevpatoriya:128.
Golovakha ML. Diagnostics, conservative treatment and reconstructive-restorative surgery of knee osteoarthritis: abstract dis. the doctor of medical sciences. Kharkiv, 2011. 36 p.
Lebets IS, Shevchenko NS, Matviienko OV. Diagnosis of osteoarthritis in adolescents: recommendations. Kyiv, 2005. 21 p.
Stefanov OV. Preclinical studies of drugs (guidelines). Kyiv: Avitsena, 2001. 297 p.
Zborovskiy AB, Zborovskaya IA. Osteoarthritis: a tutorial. Volgograd, 2010. 48 p.
Zorya VI, Lazishvili GD, Shpakovskiy DE. Deforming arthrosis of the knee: a guide. Moscow: Litera, 2010. 320 p.
Kamishnikov VS. Clinical and biochemical laboratory diagnostics. Directory. 2nd Ed. Minsk: Interpresservis, 2003. 463 p.
Kovalenko VM, Bortkevich OP. Osteoarthritis. Practical guidance. Kyiv: Morion, 2010. 608 p.
Korzh NA. Forum of experts on sequential therapy of osteoarthritis. Orthopaedics, Traumatology and Prosthetics. 2014;(3):144-5. doi: http://dx.doi.org/10.15674/0030-598720143144-145.
Zupanets IA, Korzh NA, Dedukh NV. Guidelines for experimental research and clinical studies antiarthritic (chondromodulated) medicines. Ed. Sereda PI. Kyiv, 1999. 56 p.
Sarkisov DS, Perov YuL. Microscopic technique. Guide. Moscow: Medicine, 1996. 542 p.
Moskvin SV, Buylin VA. Basics of laser therapy. Moscow: Piramida, 2006. 256 p.
Dedukh NV, Zupanets IA, Chernikh VF, Drogovoz SM. Osteoarthritis. Ways of pharmacological correction. Kharkov: Osnova, 1992. 140 p.
Korzh MO, Makolinets VI, Diedukh NV, Zupanets IA, Grashchenkova TM, Malyshkina SV, Makolinets KV. Method of complex conservative treatment of osteoarthritis of the joints of the lower extremities. Patent 60579 UА. МPK A61N 5/067, A61K 31/196, A61K 31/726 (2006.01). No u201013838; 22.11.2010; 25.06.2011. Bul. No 12.
Polivoda AN, Vishnivskiy VA. The concept of chronic pathological process in osteoarthritis. Odessa medical journal. 2009;113(3):38–40.
Lebets IS, Shevchenko NS, Matviienko OV. Prevention and treatment: Guidelines. Kyiv, 2009. 24 p.
Rebrova OYu. Statistical analysis of medical data. Application software package Statistica. Moscow: MediaSphera, 2006. 312 p.
Semeniv IP. The complex treatment of patients with early stages of gonarthritis: abstract dis. The candidate of medical sciences. Kyiv, 2005. 24 p.
Sobolevskyi YuL. Features of structural and functional abnormalities in the early stages of osteoarthritis of the knee joint (the issue of early diagnosis, treatment and prevention of progression): abstract dis. the candidate of medical sciences. Kharkiv, 2009. 20 p.
Joint: morphology, clinical features, diagnosis, treatment. Eds. Pavlova VN, Pavlov GG, Shostak NA, Slutskiy LI. Moscow, 2011. 552 p.
Herrero-Beaumont G, Ivorra JA, Trabado MC, Blanco FJ, Benito P, Martín-Mola E, Paulino J, Marenco JL, Porto A, Laffon A, Araújo D, Figueroa M, Branco J. Glucosamine sulfate in the treatment of knee osteoarthritis symptoms: A randomized, double-blind, placebo-controlled study using acetaminophen as a side comparator. Arthritis Rheum. 2007;56(2):555–67. doi: 10.1002/art.22371.
Rezende MU, Campos GC, Pailo A.F. Current concepts in osteoarthritis. Acta Ortop Bras. 2013;21(2):120–2. doi: 10.1590/S1413-78522013000200010.
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