Morphological features state of the blood supply and tissue viability in femoral and tibial bones during tourniquet limb ischemia and recirculation period

Authors

  • Valeriy Hryhorovskyi
  • Olexey Dolgopolov
  • Anastasiya Hryhorovska
  • Andrii Lysak

DOI:

https://doi.org/10.15674/0030-59872016241-47

Keywords:

tourniquet ischemia, bone marrow, compact bone, cancellous bone, dynamics ischemic changes, bone marrow regeneration, bone regeneration

Abstract

Detailed studies of histopathological changes and the state of blood supply to the tissues of the limb has not been studied yet. Application with following removing the tourniquet had not been carried out. It remains unclear topography, what are the most sensitive to ischemia parts of bone (bone and bone marrow), dynamics and pace of pathological and reparative changes.

Objective: to establish experimental features of the blood supply, the viability and the possibility of tissue regeneration of the femur and tibia in the dynamics of ischemic and post-ischemic periods.

Methods: we investigated the tissue of the femur and tibia in rabbits (of 76 limbs), in which created a tourniquet ischemia of the femur and tibia with varying duration (3 and 6 hours) followed by recirculation from 0.5 hours to 30 days. After sacrificing the animals ink-gelatin mass vascular injection performed with following histological analysis.

Results: after the tourniquet application for 3 and 6 hours we found in the bone marrow of femoral and tibial epimetaphyses violations of the microcirculation in the sinusoids and small veins, which didn't result into ischemic necrosis. Such areas observed in the individual cases and in certain anatomical bone locations.

Conclusions: in the tourniquet application on the hind limb of rabbits for 3 and 6 h,there are two different areas of circulatory disorders (compression and regional ischemia), in which tissues microcirculation and ischemic changes going off. After removing the tourniquet and blood supply recycling restoration different parts of the femur and tibia are differently sensitive to ischemia for tourniquet application for 6 hours were the primary structure of spongiosis and bone marrow of the proximal metaphyseal tibia.

References

  1. Hryhorovskii VV. Posttraumatic bone lesions: pathomorphology and pathogenesis: abstract dis. the doctor of medical science. Kharkiv, 2001. 38 p.
  2. Strafun SS, Brusko AT, Liabakh AP, et al. Prophylaxis, diagnosis and treatment of wrist and foot ischemic contractures. Kyiv: Stylos, 2007. 263 p.
  3. Grigorovskii VV. Acute traumatic ischemic bone lesions: pathogenesis, morphogenesis, differential diagnosis. J of AMS of Ukraine. 2008;(1):116–33.
  4. Grigorovskii VV. Morphology and pathogenesis of long bone infarction by experimental limb soft tissue trauma with principal nutrient artery disruption. Annals of traumatology and orthopaedics. 1997;(3–4):21–9.
  5. Brookes M, Revell WJ. Blood supply of Bone. Scientific aspects. Berlin: Springer, 1998. 359 p.
  6. Bilinskyi PI, Hryhorovskyi VV. Histological and angiomorphological features of bone blood supply condition by the usage of various design fixators after osteotomy (experimental study). Orthopaedics, Traumatology and Prosthetics. 2002;(4):80–5.
  7. Strafun SS, Brusko AT, Liskina IV, et. al. Interrelations between intraosseous andblood sub-fascial pressure. Visnyk orthopaedii, traumatologii ta protesuvannia. 2005;(2):12–5.
  8. Strafun SS, Brusko AT, Dolgopolov OV, Boier VA. Sub-fascial and intraosseous pressure changes by acute experimental limb tourniquet ischaemia. Visnyk orthopaedii, traumatologii ta protesuvannia. 2007;(4):9–13.
  9. European Convention for the protection of vertebrate animals used for experimental and other scientific purpose: Council of Europe, Strasbourg, 18.03.1986. Strasbourg, 1986. 52 p.
  10. Beliaeva AA, Barer FS, Semionova GA. The method of intravital animal locomotor apparatus vascular mesh injection with india ink-gelatinous mixture. Patterns of spine and extremities supporting structures morphogenesis on various stages of ontogenesis. Yaroslavl, 1985, pp.64–9.
  11. Grigorovskii VV. Morphological dynamics and correlation-regression dependences of damage and regeneration morphometric indices in experimental embolic bone infarction. Morphology. 1999;6:54–58.
  12. Grigorovskii VV, Magomedov S. Histologic and angiomorphologic changes dynamics in experimental long bone traumatic infarction. Orthopaedics, Traumatology and Prosthetics. 1996;(1):30–5.
  13. Grigorovskii VV. The quantitative pathomorphologic changes dynamics and problems of long bone traumatic infarction pathogenesis (experimental study). Orthopaedics, Traumatology and Prosthetics. 1999;(2):83–7.

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How to Cite

Hryhorovskyi, V., Dolgopolov, O., Hryhorovska, A., & Lysak, A. (2016). Morphological features state of the blood supply and tissue viability in femoral and tibial bones during tourniquet limb ischemia and recirculation period. ORTHOPAEDICS TRAUMATOLOGY and PROSTHETICS, (2), 41–47. https://doi.org/10.15674/0030-59872016241-47

Issue

No. 2 (2016)

Section

ORIGINAL ARTICLES

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Copyright (c) 2016 Valerii Hryhorovskyi, Oleksii Dolgopolov, Anastasiia Hryhorovska, Andrii Lysak

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