Morphological peculiarities of bone healing in the place of experimental cortical defect of long bone of rats in the conditions of naturalhydroxyapatite implantation


  • Olexiy Korenkov



Cerabone®, hydroxyapatite, reparative osteogenesis, compact bone


The goal: to investigate bone-healing process after implantation of osteoplastic material Cerabone® in compact bone defect.

Methods: experiment was performed on 24 white rats (male). Hole-like defect (2.5 mm diameter) was created on the middle third of femur diaphysis using portable drill with spheric mill at low speed with cooling. The defect reached intramedullary canal; it was filled with osteoplastic material Cerabone® in experimental animals and was left unfilled in control. Bone fragments were studied on the 15th and 30th days using method of light microscopy with morphometry and scanning electron microscopy that was performed on electronic microscope «РЭМ 106-И». Morphometric analysis was carried out using image-processing programs «Видео-тест» and «Видео-размер».

Results: it was found out that material Cerabone® did not provoke any inflammatory reaction. The lacunas with typical osteocytes were located in maternal bone adjacent to the site of transplantation. This fact gives the evidence of material biocompatibility. At all stages of study the signs of desmal osteogenesis only were identified, the presence of bone and connective tissues in defects in both groups pointed on this fact. Bone tissue of regenerate in animals of experimental and control groups on the 15th day took the form of large-loop and small-looped network of trabeculas. On 30th day the areas of bone similar in structure to maternal bone were found. The forming of bone regenerate with high density of osteoblasts and osteocytes was found in the animals of experimental group only on outer side of Cerabone® surface without penetration into osteoplastic material. The entire period of observation Cerabone® provided the stable volume of defect due to good integration with bone tissue of regenerate and absence of reliable signs of resorption.


Grigorian AS, Toporkova AK. Problems of integration of implants in bone tissue (theoretical aspects). Moscow: Technosphere, 2007. 128 p.

McKinnis LynnN. Radial diagnostics in traumatology and orthopedics. Clinical guidelines. Moskow: Izdatelstvo Panfilova, 2015. 644 p.

Osipenkova-Vichtomova T. K. Histomorphological examination of the bones. Moskow: JSC «Publishing house «Medicine», 2009. 152 p.

Pankratov AS, Lekishvili MV, Kopecky IS. Bone grafting in dentistry and maxillofacial surgery. Osteoplastic materials: A Guide for Physicians. Moscow: BINOM, 2011. 272 p.

Sevastyanov VI, Kirpichnikov MP. Biocompatible Materials. Moskow: LLC «Publisher «Medical News Agency», 2011. 544 p.

Khalafyan AA. STATISTICA 6. Mathematical Statistics with elements of probability theory. Moskow: BINOM Publishing, 2011. 496 p.

Artzi Z, Wasersprung N, Weinreb M, Steigmann M, Prasad HS, Tsesis I. Effect of guided tissue regeneration on newly formed bone and cementum in periapical tissue healing after endodontic surgery: an in vivo study in the cat. J Endodontics. 2012;38(2):163–9, doi: 10.1016/j.joen.2011.10.002.

Huber FX, Berger I, McArthur N, Huber C, Kock HP, Hillmeier J, Meeder PJ Evaluation of a novel nanocrystalline hydroxyapatite paste and a solid hydroxyapatite ceramic for the treatment of critical size bone defects (CSD) in rabbits. J Mater. Sci. Mater. Med. 2008;19(1):33–8.

Mordenfeld A, Hallman M, Johansson CB, Albrektsson T. Histological and histomorphometrical analyses of biopsies harvested 11 years after maxillary sinus floor augmentation with deproteinized bovine and autogenous bone Clin Oral Implants Res. 2010;21(9):961–70. doi: 10.1111/j.1600-0501.2010.01939.x.

Riachi F, Naaman N, Tabarani C, Aboelsaad N, Aboushelib MN, Berberi A, Salameh Z Influence of material properties on rate of resorption of two bone graft materials after sinus lift using radiographic assessment. Int J Dent. 2012; 2012:737262.. doi: 10.1155/2012/737262.

Seidel P, Dingeldein E. Cerabone® — eine Spongiosa-Keramik bovinen Ursprungs. Materialwissenschaft und Werkstofftechnik. 2004;35(4):208–12.

Rothamel D, Schwarz F, Smeets R, Happe A, Fienitz T, Mazor Z, Zöller J. Sinus floor elevation using a sintered, natural bone mineral. A histological case report study. Int J Oral Maxillofac Implants. 2012;27(1):146–54.

Tadic DA, Epple M. thorough physicochemical characterisation of 14 calcium phosphate-based bone substitution materials in comparison to natural bone. Biomaterials. 2004;25:987–94.