Clonogenic activity of bone marrow stromal stem cells in bones of the elbow joint in patients with rheumatoid arthritis
Keywords:rheumatoid arthritis, upper extremity, clonogenic bone marrow activity
Rheumatoid arthritis (RA) affects about 1 % of the world population. In the debut of the disease of the joints lesions of the upper extremity they observe in 70 % of cases, and the elbow joint — in 5–20 %. Objective: To study the regenerative potential of bone tissue in RA patients with lesions of the upper extremities and on the basis of study of activity of bone marrow stromal stem cells (BMSSC) to justify tactics of surgical treatment. Methods: cultural, statistical. Results: The growth of colonies of BMSSC in RA patients with lesions of the elbow joint registered only in 5.9 % of cases. The cloning efficiency of fibroblast colony-forming units of bone marrow of the humerus in RA was only 0.33 which is 20 times lower than in analogous one for the fracture of the humerus — (32,7 ± 2,5). Besides this 1 sm3 of spongiosa of the humerus contains nucleated cells 2.9 times greater (p < 0.05) than in the ulna (0,315 ± 0,111 vs. 0,110 ± 0,021, respectively). When cloning BMSSC bacterial pro-growth recorded an average in 47 % (8 of 17) cultures of spongiosa of the humerus and ulna. Satisfactory outcomes of surgery (biological arthroplasty and corrective osteotomy) for restoration function of the upper extremity in RA can not be provided by BMSSC spongiosa of the humerus and ulna with low clonogenic activity. Conclusion: In patients with RA clonogenic activity of BMSSC of the humerus and ulna is sharply reduced. Contamination by microorganisms of periarticular tissues and spongiosa of the bones of the elbow joint significantly hinders the process of bone remodeling in patients with RA. Results of this study allow us to make a tentative conclusion that the arthroplasty is the most promising method of orthopedic treatment of RA patients with lesions of the elbow joint which will allow to restore function of the upper limb in better way and to improve quality of life in these patients.
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Copyright (c) 2014 Sergey Gerasimenko, Lesya Panchenko, Andrey Babko
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