BONE REGENERATION AFTER IMPLANTATION OF CALCIUM PHOSPHATE CEMENTS BASED ON METASTABLE TRICALCIUM PHOSPHATE (IN VIVO EXPERIMENTAL STUDY)

Authors

  • Karolina Poplavska Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of Ukraine, Kharkiv, Ukraine
  • Nataliya Ashukina Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of Ukraine, Kharkiv, Ukraine https://orcid.org/0000-0002-0478-7440

DOI:

https://doi.org/10.15674/0030-59872023141-48

Keywords:

Bone defect, bone repair, calcium phosphate cement, metastable αʹ‒tricalcium phosphate, hydroxyapatite, rat femur, experiment

Abstract

Calcium phosphatCalcium phosphate cement (CPC) is a material used to fill bone defects. Its advantages include being able to fill irregularly shaped spaces, its similarity to bone tissue, and ease of biodegradation. However, insufficient durability and unpredictable rate of resorption limit CPC use. Objective. Study the dynamics of morphological changes in rat femurs after implanting two types of CPC based on metastable αʹ‒tricalcium phosphate
(αʹ‒TCP) into defects in the distal metaphysis. Methods. 42 male white rats were used in the study. In each rat, defects were created in the distal metaphysis of the left femur and filled with one of the two types of CPC. The animals were split into two groups: І (n = 21) — CPC based on αʹ‒TCP powder; ІІ (n = 21) — CPС based on αʹ‒TCP powder reinforced with hydroxyapatite (HA) whiskers (4 % mass). Both varieties of CPC were developed and prepared at the Department of Solid-State Physics at the V. N. Karazin Kharkiv National University (Ukraine). 14, 30, and 60 days after the surgery, the animals were sacrificed, and histological analyses were performed. Results. For both types of CPC, inflammation was not observed in the region around the implant at 14, 30, or at 60 days. Bone tissue formed on the surface of the materials. The stages of bone repair were similar to the known stages of bone repair. As a result of the resorption of the CPC, 60 days after surgery the CPC comprised 26.83 % of the area of the defect in group I and 29.93 % in group II. The rest of the area was composed of lamellar bone. The two groups did not differ significantly in rate of CPC resorption or bone tissue formation. Conclusions. The two types of CPC studied, based on αʹ‒TCP (group I) and αʹ‒TCP reinforced with HA whiskers (group II), are biocompatible, osteoconductive, and osteoinductive. In addition, these materials are biodegradable and, with time, are replaced by bone tissue.

Author Biographies

Karolina Poplavska, Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of Ukraine, Kharkiv

MD

Nataliya Ashukina, Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of Ukraine, Kharkiv

PhD in Biol. Sci

References

  1. Zyman, Z. Z. (2018). Calcium-phosphate biomaterials. Textbook, Kharkiv. (in Ukrainian)
  2. Filipenko, V., Bondarenko, S., Mezentsev, V., & Ashukina, N. (2011). Use of modern biomaterials for osteoplasty of acetabular defects in hip joint arthroplasty. ORTHOPAEDICS, TRAUMATOLOGY and PROSTHETICS, (4), 24. https://doi.org/10.15674/0030-59872011424-28 (in russian)
  3. Eliaz, N., & Metoki, N. (2017). Calcium Phosphate Bioceramics: A Review of Their History, Structure, Properties, Coating Technologies and Biomedical Applications. Materials, 10(4), 334. https://doi.org/10.3390/ma10040334
  4. Samavedi, S., Whittington, A. R., & Goldstein, A. S. (2013). Calcium phosphate ceramics in bone tissue engineering: A review of properties and their influence on cell behavior. Acta Biomaterialia, 9(9), 8037–8045. https://doi.org/10.1016/j.actbio.2013.06.014
  5. Bohner, M., Santoni, B. L. G., & Döbelin, N. (2020). β-tricalcium phosphate for bone substitution: Synthesis and properties. Acta Biomaterialia, 113, 23–41. https://doi.org/10.1016/j.actbio.2020.06.022
  6. Hernigou, P., Dubory, A., Pariat, J., Potage, D., Roubineau, F., Jammal, S., & Flouzat Lachaniette, C. H. (2017). Beta-tricalcium phosphate for orthopedic reconstructions as an alternative to autogenous bone graft. Morphologie, 101(334), 173–179. https://doi.org/10.1016/j.morpho.2017.03.005
  7. Bei, T., Yang, L., Huang, Q., Wu, J., & Liu, J. (2022). Effectiveness of bone substitute materials in opening wedge high tibial osteotomy: a systematic review and meta-analysis. Annals of Medicine, 54(1), 565–577. https://doi.org/10.1080/07853890.2022.2036805.
  8. Ambard, A. J., & Mueninghoff, L. (2006). Calcium Phosphate Cement: Review of Mechanical and Biological Properties. Journal of Prosthodontics, 15(5), 321–328. https://doi.org/10.1111/j.1532-849x.2006.00129.x
  9. Korzh, M., Filipenko, V., Poplavska, K., & Ashukina, N. (2021). Materials based o tricalcium phosphate as bone defects substitute (literature review). ORTHOPAEDICS, TRAUMATOLOGY and PROSTHETICS, (2), 100–107. https://doi.org/10.15674/0030-598720212100-107
  10. Ginebra, M.-P., Canal, C., Espanol, M., Pastorino, D., & Montufar, E. B. (2012). Calcium phosphate cements as drug delivery materials. Advanced Drug Delivery Reviews, 64(12), 1090–1110. https://doi.org/10.1016/j.addr.2012.01.008
  11. Yousefi, A.-M. (2019). A review of calcium phosphate cements and acrylic bone cements as injectable materials for bone repair and implant fixation. Journal of Applied Biomaterials & Functional Materials, 17(4), 228080001987259. https://doi.org/10.1177/2280800019872594
  12. Carrodeguas, R. G., & De Aza, S. (2011). α-Tricalcium phosphate: Synthesis, properties and biomedical applications. Acta Biomaterialia, 7(10), 3536–3546. https://doi.org/10.1016/j.actbio.2011.06.019
  13. Schröter, L., Kaiser, F., Stein, S., Gbureck, U., & Ignatius, A. (2020). Biological and mechanical performance and degradation characteristics of calcium phosphate cements in large animals and humans. Acta Biomaterialia, 117, 1–20. https://doi.org/10.1016/j.actbio.2020.09.031.
  14. O'Neill, R., McCarthy, H. O., Montufar, E. B., Ginebra, M. P., Wilson, D. I., Lennon, A., & Dunne, N. (2017). Critical review: Injectability of calcium phosphate pastes and cements. Acta Biomaterialia, 50, 1–19. https://doi.org/10.1016/j.actbio.2016.11.019
  15. Dapporto, M., Tavoni, M., Restivo, E., Carella, F., Bruni, G., Mercatali, L., Visai, L., Tampieri, A., Iafisco, M., & Sprio, S. (2022). Strontium-doped apatitic bone cements with tunable antibacterial and antibiofilm ability. Frontiers in Bioengineering and Biotechnology, 10. https://doi.org/10.3389/fbioe.2022.969641.
  16. Cai, P., Lu, S., Yu, J., Xiao, L., Wang, J., Liang, H., Huang, L., Han, G., Bian, M., Zhang, S., Zhang, J., Liu, C., Jiang, L., & Li, Y. (2023). Injectable nanofiber-reinforced bone cement with controlled biodegradability for minimally-invasive bone regeneration. Bioactive Materials, 21, 267–283. https://doi.org/10.1016/j.bioactmat.2022.08.009
  17. On protection of animals from cruel treatment: Law of Ukraine №3447-IV of February 21, 2006. The Verkhovna Rada ofUkraine. (In Ukrainian). URL: http://zakon.rada.gov.ua/cgi-bin/laws/ main.cgi?nreg=3447-15
  18. European Convention for the protection of vertebrate animals used for research and other scientific purposes. Strasbourg, 18 March 1986: official translation. Verkhovna Rada of Ukraine. (In Ukrainian). URL: http://zakon.rada.gov.ua/cgi-bin/laws/ main.cgi?nreg=994_137.21.
  19. Zyman, Z., Goncharenko, A., Khavroniuk, O., & Rokhmistrov, D. (2020). Crystallization of metastable and stable phases from hydrolyzed by rinsing precipitated amorphous calcium phosphates with a given Ca/P ratio of 1:1. Journal of Crystal Growth, 535, 125547. https://doi.org/10.1016/j.jcrysgro.2020.125547
  20. Goncharenko, A., Zyman, Z., Epple, M. & [et al.] (2020).Structure-property relationships in a reinforced calcium phosphate cement based on metastable α'-tricalcium phos¬phate. Joint Polish-German Crystallographic Meeting, Book of abstracts. Wroclaw, Poland.
  21. Zyman, Z., Epple, M., Glushko, V. & [et al.] (2006). Hydroxyapatite whiskers by hydrothermal synthesis. Biomaterialen, 7 (3), 252.
  22. Popsuyshapka, O., Litvishko, V., & Ashukina, N. (2015). Clinical and morphological stages of bone fragments fusion. ORTHOPAEDICS, TRAUMATOLOGY and PROSTHETICS, (1), 12. https://doi.org/10.15674/0030-59872015112-20 (in Ukrainian)
  23. Rojbani, H., Nyan, M., Ohya, K., & Kasugai, S. (2011). Evaluation of the osteoconductivity of α-tricalcium phosphate, β-tricalcium phosphate, and hydroxyapatite combined with or without simvastatin in rat calvarial defect. Journal of Biomedical Materials Research Part A, 98A(4), 488–498. https://doi.org/10.1002/jbm.a.33117
  24. Tronco, M. C., Cassel, J. B., & dos Santos, L. A. (2022). α-TCP-based Calcium Phosphate Cements: a critical review. Acta Biomaterialia. https://doi.org/10.1016/j.actbio.2022.08.040

Downloads

How to Cite

Poplavska, K. ., & Ashukina, N. . (2023). BONE REGENERATION AFTER IMPLANTATION OF CALCIUM PHOSPHATE CEMENTS BASED ON METASTABLE TRICALCIUM PHOSPHATE (IN VIVO EXPERIMENTAL STUDY). ORTHOPAEDICS TRAUMATOLOGY and PROSTHETICS, (1), 41–48. https://doi.org/10.15674/0030-59872023141-48

Issue

No. 1 (2023)

Section

ORIGINAL ARTICLES

License

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

The authors retain the right of authorship of their manuscript and pass the journal the right of the first publication of this article, which automatically become available from the date of publication under the terms of Creative Commons Attribution License, which allows others to freely distribute the published manuscript with mandatory linking to authors of the original research and the first publication of this one in this journal.

Authors have the right to enter into a separate supplemental agreement on the additional non-exclusive distribution of manuscript in the form in which it was published by the journal (i.e. to put work in electronic storage of an institution or publish as a part of the book) while maintaining the reference to the first publication of the manuscript in this journal.

The editorial policy of the journal allows authors and encourages manuscript accommodation online (i.e. in storage of an institution or on the personal websites) as before submission of the manuscript to the editorial office, and during its editorial processing because it contributes to productive scientific discussion and positively affects the efficiency and dynamics of the published manuscript citation (see The Effect of Open Access).