Histological analysis of vertebral disc herniations in patients with different age groups

Authors

  • Volodymyr Radchenko Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of Ukraine, Kharkiv, Ukraine https://orcid.org/0000-0001-5949-0882
  • Valentyn Piontkovsky CHI «Rivne Regional Clinical Hospital». Ukraine , Ukraine
  • Ninel Dedukh SI "D.F.Chebotarev Institute of Gerontology of the NAMS of Ukraine", Kyiv , Ukraine https://orcid.org/0000-0003-0307-2328

DOI:

https://doi.org/10.15674/0030-59872018233-43

Keywords:

herniation of vertebral disc, structure, age changes

Abstract

Studying of degenerative changes in the structure of vertebral discs and peculiarities of microscopic organization in patients with different age groups gives us possibility to connect pathomorphological changes with clinical symptoms.

Objective: to study the peculiarities of herniations structure which were obtained after surgeries in patients of different age groups.

Methods: herniations of vertebral discs on the levels LIII–LIV, LIV–LV и LV–SI were obtained after surgeries of 4 3 patients (24 women, 19 men) of three age groups: the 1st — 25–44 y. o, the 2nd — 45– 60, the 3rd — 61–75. We used histology with semiquantitative estimation (score) of degenerative changes.

Results: it was shown that the sex has not influence on the severity of degenerative changes of herniations. According to some histological signs we concluded distinctive features. It was found that in the 1st group in the samples there were mostly chondrons which contained more than 15 chondrocytes with large cores, it testifies of proliferation and hypertrophy. Severe destructive lesions of matrix were not found. With aging degenerative changes in herniations are progressed. So the number of large chordons was in 1.7 and 1.4 times lower in the 2nd group compare to the 1st one. Fibroblasts accumulation increased in the matrix (in 3 and 3.2 times in men and women), small foci of granular destruction and chondrogenesis appeared, areas with increased dense of fibroblasts, small bone sequestrations (21.4 %). In the 3rd group in men and women the number of chondrons in herniations were decreased in 2.6 and 2.3 times compare to the 1st group. Areas of defibration in the matrix became wider in 2.4 and 2 times, granular destruction — in 1.3 and 1.4 times. Large areas were with chondrogenesis and in 78.6 % of cases we have found small bone fragments.

Conclusion: with aging the severity of destructive changes in herniations increase significantly.

Author Biographies

Volodymyr Radchenko, Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of Ukraine, Kharkiv

MD, Prof. in Traumatology and Orthopaedics 

 

Valentyn Piontkovsky, CHI «Rivne Regional Clinical Hospital». Ukraine 

PhD in Traumatology and Orthopaedics 

 

Ninel Dedukh, SI "D.F.Chebotarev Institute of Gerontology of the NAMS of Ukraine", Kyiv 

Dr. Biol. Sci., Prof.

 

References

  1. Deyo, R. A., & Mirza, S. K. (2016). Herniated lumbar intervertebral disk. New England Journal of Medicine, 374, 1763–1772. doi: https://doi.org/10.1056/NEJMcp1512658
  2. Radchenko, V., Piontkovsky, V., Kosterin, S., & Dedukh, N. (2018). Intervertebral disc: regeneration, herniation formation stages and molecular profile (literature review). Orthopaedics, traumatology and prosthetics, 4, 99–106. doi: https://doi.org/10.15674/0030-59872017499-106 (in Russian)
  3. Postacchini, F., & Postacchini, R. (2011). Operative management of lumbar disc herniation: the evolution of knowledge and surgical techniques in the last century. Acta neurochirurgica's Supplement, 108, 17–21. doi: https://doi.org/10.1007/978-3-211-99370-5_4
  4. Pouriesa, M., Fouladi, R. F., & Mesbahi, S. (2013). Disproportion of end plates and the lumbar intervertebral disc herniation. The Spine Journal, 13(4), 402-407. doi: https://doi.org/10.1016/j.spinee.2012.11.047
  5. Cai, Z., Ma, D., Li, F., Chen, R., Liu, Z., Zhang, Z., … Lu, X. (2013). Trend of the incidence of lumbar disc herniation: decreasing with aging in the elderly. Clinical Interventions in Aging, 1047. doi: https://doi.org/10.2147/cia.s49698
  6. Nilsson, E., Brisby, H., Rask, K., & Hammar, I. (2013). Mechanical compression and nucleus pulposus application on dorsal root ganglia differentially modify evoked neuronal activity in the thalamus. BioResearch Open Access, 2(3), 192-198. doi: https://doi.org/10.1089/biores.2012.0281
  7. Adams, M. A., & Dolan, P. (2016). Lumbar intervertebral disk injury, herniation and degeneration. Advanced Concepts in Lumbar Degenerative Disk Disease, 23-39. doi: https://doi.org/10.1007/978-3-662-47756-4_3
  8. Baptista, J. D., Fontes, R. B., & Liberti, E. A. (2015). Aging and degeneration of the intervertebral disc: review of basic science. Coluna/Columna, 14(2), 144-148. doi: https://doi.org/10.1590/s1808-185120151402141963
  9. Kotwal, S., Mohan, H., Bahadur, R., & Bal, A. (2002). A clinicopathological study of changes in intervertebral discs. The Internet Journal of Pathology, 2(2), 1–6.
  10. Lama, P., Zehra, U., Balkovec, C., Claireaux, H. A., Flower, L., Harding, I. J., … Adams, M. A. (2014). Significance of cartilage endplate within herniated disc tissue. European Spine Journal, 23(9), 1869-1877. doi: https://doi.org/10.1007/s00586-014-3399-3
  11. Oprea, M., Popa, I., Cimpean, A. M., Raica, M., & Poenaru, D. V. (2015). Microscopic assessment of degenerated intervertebral disc: clinical implications and possible therapeutic challenge. Іn vivo, 29(1), 95–102.
  12. ST/ESA/STAT/SER.M/74. Рrovisional guidelines on standard international age classifications. (1982). New York.
  13. Sarkisov, D. S., & Perov, Yu. L. (1996). Microscopic technology. Moscow: Medicine. (in Russian)
  14. Boos, N., Weissbach, S., Rohrbach, H., Weiler, C., Spratt, K. F., & Nerlich, A. G. (2002). Classification of age-related changes in lumbar intervertebral discs: 2002 Volvo Award in basic science. Spine, 27 (23), 2631-2644. doi: https://doi.org/10.1097/01.BRS.0000035304.27153.5B
  15. Weiler, C., Lopez-Ramos, M., Mayer, H., Korge, A., Siepe, C. J., Wuertz, K., … Nerlich, A. G. (2011). Histological analysis of surgical lumbar intervertebral disc tissue provides evidence for an association between disc degeneration and increased body mass index. BMC Research Notes, 4(1), 497. doi: https://doi.org/10.1186/1756-0500-4-497
  16. Lama, P., Le Maitre, C. L., Dolan, P., Tarlton, J. F., Harding, I. J., & Adams, M. A. (2013). Do intervertebral discs degenerate before they herniate, or after? The Bone & Joint Journal, 95-B(8), 1127-1133. doi: https://doi.org/10.1302/0301-620x.95b8.31660
  17. Hamdan, T. A., & Jbara, K. K. (2006). Histological and structural study of prolapsed intervertebral disc. Basrah Journal of Surgery.
  18. Dedukh, N. V. (2010). Vertebral column. Guide to histology (2 nd ed.) (pp. 301-306). In R.K. Danilov (Ed.). St Petersburg: Spec-Lit. (in Russian)
  19. Dowdell, J., Erwin, M., … Choma, T. (2017). Intervertebral disc degeneration and reparation. Neurosurgery, 80(3S), S46–S54. doi: https://doi.org/10.1093/neuros/nyw078
  20. Roberts, S. (2002). Disc morphology in health and disease. Biochemical Society Transactions, 30(6), 864-869. doi: https://doi.org/10.1042/bst0300864
  21. Johnson, W. E., Caterson, B., Eisenstein, S. M., Hynds, D. L., Snow, D. M., & Roberts, S. (2002). Human intervertebral disc aggrecan inhibits nerve growth in vitro. Arthritis & Rheumatism, 46(10), 2658-2664. doi: https://doi.org/10.1002/art.10585
  22. Repanti, M., Korovessis, P. G., Stamatakis, M. V., Spastris, P., & Kosti, P. (1998). Evolution of disc degeneration in lumbar spine. Journal of Spinal Disorders, 11(1), 41–45. doi: https://doi.org/10.1097/00002517-199802000-00007
  23. Rajasekaran, S., Bajaj, N., Tubaki, V., Kanna, R. M., & Shetty, A. P. (2013). ISSLS Prize Winner. Spine, 38(17), 1491-1500. doi: https://doi.org/10.1097/brs.0b013e31829a6fa6
  24. Willburger, R. E., Ehiosun, U. K., Kuhnen, C., Krämer, J., & Schmid, G. (2004). Clinical symptoms in lumbar disc herniations and their correlation to the histological composition of the extruded disc material. Spine, 29(15), 1655-1661. doi: https://doi.org/10.1097/01.brs.0000133645.94159.64

Downloads

How to Cite

Radchenko, V., Piontkovsky, V., & Dedukh, N. (2023). Histological analysis of vertebral disc herniations in patients with different age groups. ORTHOPAEDICS TRAUMATOLOGY and PROSTHETICS, (2), 33–43. https://doi.org/10.15674/0030-59872018233-43

Issue

No. 2 (2018)

Section

ORIGINAL ARTICLES

License

Copyright (c) 2018 Volodymyr Radchenko, Valentyn Piontkovsky, Ninel Dedukh

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

The authors retain the right of authorship of their manuscript and pass the journal the right of the first publication of this article, which automatically become available from the date of publication under the terms of Creative Commons Attribution License, which allows others to freely distribute the published manuscript with mandatory linking to authors of the original research and the first publication of this one in this journal.

Authors have the right to enter into a separate supplemental agreement on the additional non-exclusive distribution of manuscript in the form in which it was published by the journal (i.e. to put work in electronic storage of an institution or publish as a part of the book) while maintaining the reference to the first publication of the manuscript in this journal.

The editorial policy of the journal allows authors and encourages manuscript accommodation online (i.e. in storage of an institution or on the personal websites) as before submission of the manuscript to the editorial office, and during its editorial processing because it contributes to productive scientific discussion and positively affects the efficiency and dynamics of the published manuscript citation (see The Effect of Open Access).