Modelling of an inflammatory and destructive process in peripheral nerves

Authors

DOI:

https://doi.org/10.15674/0030-59872012379-83

Keywords:

peripheral nerves, rats, transplantation, nucleus pulposus

Abstract

The article contains data of a morphological analysis by results of transplantation of an autologous vertebral pulp of white rats to the tibial branch of the sciatic nerve. It is shown that on the 15th day after the reproduction of this model the nervous trunk and perineural tissues of animals developed signs of a chronic inflammatory-destructive process. Destruction, vacuolization and fragmentation of the myelin sheath, destruction foci in nonmyelinated nerve fibres, nuclear heterochromatization and a low density of membrane organoids in the cytoplasm of Schwann’s cells were revealed on the ultrastructural level.

References

  1. Волков А. В. Экспериментальные модели дегенеративных заболеваний межпозвонковых дисков / А. В. Волков // Хирургия позвоночника. — 2007. — № 4. — С. 41–46.
  2. Are animal models useful for studying human disc disorders/degeneration? / M. Alini, S. M. Eisenstein, K. Ito et al. // Eur. Spine J. — 2008. — Vol. 17. — P. 2–19.
  3. Cavanaugh J. M. Neural mechanisms of lumbar pain / J. M. Cavanaugh // Spine. — 1995. — Vol. 16. — P. 1804–1809.
  4. Kulisch S. D. The tissue origin of low-back pain and sciatica: a report of pain response to tissue stimulation during operations on the lumbar spine using local anesthesia / S. D. Kulisch, C. L. Ulstrom, C. J. Michael // Orthop. Clin. North. Am. — 1991. — Vol. 22. — P. 181–187.
  5. Comparison of neuropathic pain induced by the application of normal and mechanically compressed nucleus pulposus to lumbar nerve roots in the rat / M. Kawakami, H. Hashizume, H. Nishi et al. // J. Orthop. Res. — 2003. — Vol. 21. — P. 535–539.
  6. Chronic inflammation and compression of the dorsal root contribute to sciatica induced by the intervertebral disc herniation in rats / S. X. Hou, J. G. Tang, H. S. Chen, J. Chen // Pain. — 2003. — Vol. 105. — Р. 255–264.
  7. Экспериментальные аспекты моделирования грыжи межпозвонкового диска (аналитический обзор литературы) / В. А. Радченко, Н. В. Дедух, Л. М. Бенгус, М. В. Шимон // Боль, суставы, позвоночник. — 2011. — № 2. — С. 88–93.
  8. Olmarker K. Autologous nucleus pulposus induces neurophysiologic and histologic changes in porcine cauda equina nerve roots / K. Olmarker, B. Rydevik, C. Nordborg // Spine. — 1993. — Vol. 18. — P. 1425-1432.
  9. Mechanical and biochemical injury of spinal nerve roots: a morphological and neurophysiological study / M. Cornefjord, K. Olmarker, R. Rydevik, C. Nordborg // Eur. Spine J. — 1996. — Vol. 5. — P. 187–192.
  10. Дєдух Н. В. Вплив драглистого ядра міжхребцевого диска на великогомілкову гілку сідничного нерва / Н. В. Дєдух, С. В. Малишкіна, М. В. Шимон // — Український медичний альманах. — 2011. — Т. 14, № 4. — С. 42–45.
  11. Європейська конвенція про захист хребетних тварин, що використовуються для дослідних та інших наукових цілей. Страсбург, 18 березня 1986 року: офіційний переклад [Електронний ресурс] — Режим доступу: http://zakon.rada.gov.ua/ cgi-bin/laws/main.cginreg=994_137.
  12. Саркисов Д. С. Микроскопическая техника / Д. С. Саркисов, Ю. Л. Перова. — М.: Медицина, 1996. — 542 с.
  13. Уикли Б. Электронная микроскопия для начинающих / Б. Уикли. — М.: Мир, 1975. — 324 с.
  14. Reynolds E. S. The use of lead citrate at high ph an electrono-paque stain in electron microscopy / E. S. Reynolds // J. Cell Biol. — 1963. — Vol. 17. — P. 208–212.
  15. Боровский М. Л. Регенерация нерва и трофика / М. Л. Боровский. — М.: Изд-во Академии мед. наук, 1952. — 224 с.
  16. Морфологічні зміни сідничного нерва після його ушкодження шляхом електрокоагуляції (експериментальне дослідження) / О. І. Продан, Л. М. Бенгус, О. А. Сиренко, А. І. Попов // Науковий вісник Ужгородського університету. — 2007. — Вип. 32. — С. 149–152. — (Серія «Медицина»).
  17. Mumenthaler M. Laesionen peripher nerven und radikulare syndrome / M. Mumenthaler, H. Schliack, M. Stohr. — Auflage, Stuttgart: Thieme, 1998. — P. 261–368.

Downloads

How to Cite

Bengus, L., Dedukh, N., & Shymon, M. (2012). Modelling of an inflammatory and destructive process in peripheral nerves. ORTHOPAEDICS TRAUMATOLOGY and PROSTHETICS, (3), 79–83. https://doi.org/10.15674/0030-59872012379-83

Issue

No. 3 (2012)

Section

ORIGINAL ARTICLES

License

Copyright (c) 2014 Lyudmila Bengus, Ninel Diedukh, Mykhaylo Shimon

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

The authors retain the right of authorship of their manuscript and pass the journal the right of the first publication of this article, which automatically become available from the date of publication under the terms of Creative Commons Attribution License, which allows others to freely distribute the published manuscript with mandatory linking to authors of the original research and the first publication of this one in this journal.

Authors have the right to enter into a separate supplemental agreement on the additional non-exclusive distribution of manuscript in the form in which it was published by the journal (i.e. to put work in electronic storage of an institution or publish as a part of the book) while maintaining the reference to the first publication of the manuscript in this journal.

The editorial policy of the journal allows authors and encourages manuscript accommodation online (i.e. in storage of an institution or on the personal websites) as before submission of the manuscript to the editorial office, and during its editorial processing because it contributes to productive scientific discussion and positively affects the efficiency and dynamics of the published manuscript citation (see The Effect of Open Access).