Morphological features osteointegration of porous tantalum implants in rats

Ninel Dedukh, Stanislav Bondarenko, Volodymyr Filipenko, Inna Batura

Abstract


Among the metallic materials, porous tantalum attracts grow­ing attention of orthopedics and traumatologists. The purpose of the study is to investigate osseointegration by using a porous tantalum implants in rats with normal bone health and osteopo­rosis simulated background.

Methods:the study was performed in 18 laboratory rats weighing 250–350 g. Osteoporosis modeled in 9 animals with the help of ovariectomy in 3 months prior to implantation. 3 mm diameter defect produced in the distal femoral metaphysis in normal rats (control) and with osteopo­rosis (experimental), porous tantalum pins implanted. After 14, 30 and 90 days postoperatively the material was examined by using a histology techniques.

Re­sults:it was found that the orientation of osteoreparation pro­cess was identical for both groups of animals, ie at all stages of monitoring, the formation of bone around the implant. However, in comparison with the control group osteoporosis animals had larger areas of direct contact with the tantalum implant intertrabecular spaces of the bone marrow and the formation of the parent bone connective tissue sections. In control and ex­perimental animals 90 days postoperatively bone tissue formed around the implant was presented collagen type I with a different refraction of collagen fibers. It is proved a good biologi­cal compatibility of porous tantalum implants with bone tissue and bone marrow, as well as higher quality of osseointegration. In animals with a normal structure of the bone implant 73.1 % of the perimeter was surrounded by mature bone tissue. In rats with osteoporosis this value was also quite high and amount­ed to 57.7 %.

Conclusions:the study has evidence indicating that tantalum implants can be used in orthopedics and trauma­tology in patients with normal bone health and osteoporosis.

Keywords


porous tantalum; bone regeneration; osteoporosis

References


Korzh NA, Malyshkina SV, Dedukh NV, Timchenko IB. Biomaterials in Orthopedics and Traumatology — the role of AA Korzh development problems. Heritage. Alexey Korzh: scientific and historical edition; edited by LD Goridova. Kharkiv, 2014. рр. 35-49. (in Russian)

Dedukh NV, Pobel EA. The bone tissue at norm and in osteoporosis: preparations of calcium and vitamin d (a review of literature). Orthopedics, Traumatology and Prosthetics. 2013;(3):92-8. doi: 10.15674/0030-59872013392-98. (in Russian)

European convention for the protection of vertebrate animals used for experimental and other scientific purposes. Council of Europe. Strasbourg, 18 Mar 1986.

The Law of Ukraine №3447-IV from 21.02.2006 «On protection of animals from cruelty» (Article 26).

Muzichenko PF. Materials Issues in Orthopedics and Traumatology. Lіkaryu shcho praktykue. 2012;13(1):94-8. (in Russian)

Popkov AV. Biocompatible implants in traumatology and orthopaedics (A review of literature) (review). Orthopaedic Genius (Genij Ortopedii). 2014;3:94-99. (in Russian)

Sarkisov DS, Perov JL. Microscopic techniques. Moskow: Medicine, 1996. 542 p. (in Russian)

Povoroznyuk VV, Dedukh NV, Grigorieva NV, Gopkalova IV. Experimental osteoporosis. Kyiv, 2012. 228 p. (in Russian)

Wauthle R, van der Stok J, Amin Yavari S, Van Humbeeck J, Kruth JP, Zadpoor AA, Weinans H, Mulier M, Schrooten J. Additively manufactured porous tantalum implants. Acta Biomater. 2015;14:217–25. doi: 10.1016/j.actbio.2014.12.003.

Schildhauer TA, Robie B, Muhr G, Koеller M. Bacterial adherence to tantalum versus commonly used orthopedic metallic implant materials. J Orthop Trauma. 2006;20(7):476–84.

Balla VK, Bodhak S, Bose S, Bandyopadhyay A. Direct laser processing of tantalum coating on titanium for bone replacement structures. Acta Biomater. 2010;6 (6):2329–34. DOI: 10.1016/j.actbio.2009.11.021.

Junqueira LC, Bignolas G, Brentani RR. Picrosirius staining plus polarizationmicroscopy, a specific method for collagen detection in tissue sections. Histochem J. 1979;11(4):447–55.

Paganias CG, Tsakotos GA, Koutsostathis SD, Macheras GA. Osseous integration in porous tantalum implants. Indian J Orthop. 2012;46(5):505–13. doi: 10.4103/0019-5413.101032.

Matassi F, Botti A, Sirleo L, et al. Porous metal for orthopedics implants. Clin. Cases Miner. Bone Metab. 2013;10(2):111–115.

Balla VK, Bose S, Davies NM, Bandyopadhyay A. Tantalum – а вioactive мetal for implants. JOM. 2010;62(7):61–4. doi: 10.1007/s11837-010-0110-y.

Wang Q, Qiao Y, Cheng M, Jiang G, He G, Chen Y, Zhang X, Liu X Tantalum implanted entangled porous titanium promotes surface osseointegration and bone ingrowth. Sci Rep 2016;6:26248. doi: 10.1038/srep26248.




DOI: https://doi.org/10.15674/0030-5987201645-10

Refbacks

  • There are currently no refbacks.


Copyright (c) 2016 Ninel Dedukh, Stanislav Bondarenko, Volodymyr Filipenko, Inna Batura

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.