Posterior spinal fusion formation depending on different physical activity in animals

Authors

DOI:

https://doi.org/10.15674/0030-59872016255-59

Keywords:

experiment, rats, lumbar spine, posterior fusion, paravertebral mucles, swimming

Abstract

Structural violations in paravertebral muscles — spinal units stabilizators, are related to the risk factors for degenerative lumbar spane diseases. Experts pay attention to their condition after the surgical treatment — spinal fusion. However effect of muscle activity on the result of surgical treatment is not studied well. Subject: to evaluate results of posterior spinal fusion and lumbar transpedicular spinal fusion in rats depending on animals muscle activity.

Methods: experiment is carried out in 20 laboratory rats (age 5 month, weight from 430 to 500 gr.) with were divided in to four groups, 5 animals in each, depending on the physical activity level. Animals swam before and after (I), before (II), after (III) the surgery and didn't swim (IV). Vertebral bodies of adjacent LІV, LV bodies fixed using author’s method with transpedicular instrumentation and bone autoplasty. After 3 month postoperatively bone fusion formation analyzed using clinical, radiographic and histological data. Significance of interrelations between quality signs (physical activity pattern and surgical results) evaluated on the basis of contingency tables.

Results: bone fusion formation confirmed clinicaly and radiographically after 3 month postoperatively found in 60% (II group), 40% (III), 20% (IV) and 80% (I). It is observed histological signs of significant bone formation in spinous process, spreading into inter laminar spaces that joins vertebras between.

Conclusion: it is found positive effect of physical stresses (swimming) on bone fusion formation (G = 0.671642, р = 0.013097). The best results achieved in the animals group who swim with high level of physical activity (swam before and after the surgery).

References

  1. Radchenko V, Dedukh N, Malyshkina S Lumbar facet syndrome. In: Modern techniques in spine surgery. Ed. A. Bhave. New Delhi-London-Philadelphia-Panama: The Health Sciences Publisher, 2014. pp. 175-191.
  2. Adams MA, Roughley PJ. What is intervertebral disc degeneration, and what causes it? Spine. 2006; 31:2151–61.
  3. Iatridis JC, Michalek AJ, Purmessur D, Korecki CL. Localized intervertebral disc injury leads to organ level changes in structure, cellularity, and biosynthesis. Cell Mol Bioeng. 2009;2(3):437–47.
  4. Stokes IA, Iatridis JC. Mechanical conditions that accelerate intervertebral disc degeneration: overload versus immobilization. Spine. 2004;29(23):2724–32.
  5. Crossman K, Mahon M, Watson PJ, Oldham JA, Cooper RG. Chronic low back pain-associated paraspinal muscle dysfunction is not the result of a constitutionally determined «adverse» fiber-type composition. Spine. 2004;29(6):628–34.
  6. Hultman G, Nordin M, Saraste H, Ohlsèn H. Body composition, endurance, strength, cross-sectional area, and density of mm erector spinae in men with and without low back pain. J Spinal Dis. 1993:6(2):114-23.
  7. Radchenko VA, Korzh NA. Workshop on the stabilization of thoracic and lumbar spine. Kharkov: Prapor, 2004. 154 p.
  8. Khvisyuk NI, Radchenko VA, Korzh NA. Stabilization in injuries of thoracic and lumbar spine. In: The Damages of the spine and spinal cord. Kyiv: Kniga plus, 2001. 388 p.
  9. Orthopedist’ Handbook. Eds. Korzh NA, Radchenko VA. Kyiv: ООО «Doctor media», 2011. 378 p.
  10. Kotani Y, Abumi K, Sudo H, Minami A. Effect of minimally invasive lumbar posterolateral fusion using percutaneous pedicle screw on paravertebral muscle change and postoperative residual low back pain. Spine J. 2011:11(Suppl 10): S103–4. doi: 10.1016/j.spinee.2011.08.257.
  11. Gejo R, Kawaguchi Y, Kondoh T, Tabuchi E, Matsui H, Torii K, Ono T, Kimura T. Magnetic resonance imaging and histologic evidence of postoperative back muscleinjury in rats. Spine. 2000;25(8):941–6.
  12. Hu Y, Leung HB, Lu WW, Luk KDK. Histologic and electrophysiological changes of the paraspinal muscle after spinal fusion. An experimental study. Spine. 2008;33(13):1418–22. doi: 10.1097/BRS.0b013e3181753bea.
  13. Wang TY, Pao JL, Yang RS, Jang JS, Hsu WL. The adaptive changes in muscle coordination following lumbar spinal fusion. Hum Mov Sci. 2015;40:284-97. doi: 10.1016/j.humov.2015.01.002.
  14. European convention for the protection of vertebrate animals used for experimental and other scientific purposes. Council of Europe. Strasbourg, 18 Mar 1986.
  15. Radchenko V, Skidanov A, Ivanov G, Ashukina N, Levytskyi P. Modeling of fixation with using of transpedicular constructs in the lumbar spine of the rats. Orthopaedics, Traumatology and Prosthetics. 2014;(3):86–9. doi: 10.15674/0030-59872014386-89.
  16. Radchenko V, Skidanov A, Ivanov G, Steshenko V. The method of experimental interbody fusion in animals. Patent. 94502 UA. МPK А61В 17/56 (2006.01). No. u201407005; 23.06.2014; 10.11.2014, Bul. No. 21.
  17. Sarkisov DS, Perov YuL. Microscopic technics – Microskopicheskaya teknika. Moscow: Medicine, 1996. 542 p.
  18. Matsumoto T, Toyoda H, Dohzono S, Yasuda H, Wakitani S, Nakamura H, Takaoka K. Efficacy of interspinous process lumbar fusion with recombinant human bone morphogenetic protein-2 delivered with a synthetic polymer and β-tricalcium phosphate in a rabbit model. Eur Spine J. 2012;21(7):1338–45. doi: 10.1007/s00586-011-2130-x.
  19. Walsh WR, Vizesi F, Cornwall GB, Bell D, Oliver R, Yu Y. Posterolateral spinal fusion in a rabbit model using a collagen–mineral composite bone graft substitute. Eur Spine J. 2009;18(11):1610–20. doi: 10.1007/s00586-009-1034-5.
  20. Choi YS, Kim DH, Park JH, Johnstone B, Yoo JU. Effectiveness of posterolateral lumbar fusion varies with the physical properties of demineralized bone matrix. Strip Asian Spine J. 2015;9(3):433-9. doi: 10.4184/asj.2015.9.3.433.
  21. Skidanov A, Ashukina N, Danyshchuk Z, Batura I, Radchenko V. Structural features multifidus muscle of rats after transpedicular fixation of vertebrae by various conditions of physical activity. Orthopaedics, Traumatology and Prosthetics. 2015; (2):85-91. doi: http://dx.doi.org/10.15674/0030-59872015285-91.

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How to Cite

Radchenko, V., Skidanov, A., Ashukina, N., Danishchuk, Z., & Levytskyi, P. (2016). Posterior spinal fusion formation depending on different physical activity in animals. ORTHOPAEDICS TRAUMATOLOGY and PROSTHETICS, (2), 55–59. https://doi.org/10.15674/0030-59872016255-59

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No. 2 (2016)

Section

ORIGINAL ARTICLES

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Copyright (c) 2016 Volodymyr Radchenko, Artem Skidanov, Nataliya Ashukina, Zinayda Danishchuk, Petr Levytskyi

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