Structural features multifidus muscle of rats after transpedicular fixation of vertebrae by various conditions of physical activity
Keywords:multifidus muscle, histology, lumbar spine, stabilization of the vertebrae, rats
AbstractMultifidus muscles located in the middle layer of paravertebral muscles provide support for the positioning of the spine, its rotation and extension movements. Changing in their structure with age, resulting in injuries or degenerative processes lead to their dysfunction. Objective: To investigate the structural features of the multifidus muscle (MM) in rats with different physical activity after the stabilization of the vertebral bodies LІV–LV . Methods: Experiments were performed on 20 laboratory rats (aged 5 months, weight from 430 to 500 g). The animals were divided into 4 groups: swam before and after (I), before (II) and after (III) surgery, did not swim (IV). The bodies of the adjacent vertebrae LІV–LV were fixed with author pedicle construct. After 3 months after surgery with help of histology and histochemistry methods we studied MM structure at three levels – cranially (LІІІ–LІV ), at the level LІV–LV , and caudally (LV–LVІ ) from zone of surgery. Results: such changes in the structure of muscle fibers MM, as swelling, loss of cross striation and polygonal, replacing adipose tissue, and fibrous proliferation were identified. Manifestations of regeneration were associated with non-uniform density of nuclei. In rats of group II and III content of fat tissue was significantly increased, and in IV — fat and connective tissues compared to group I. Histochemical analysis revealed that the amount of type I muscle fibers in animals of group I was larger compared to the rats of group II to 2,7 % (P> 0,05), III — to 5,77 % (P < 0,05), IV — on 11,12 % (P < 0,01), indicating positive influence of motor activity (swimming regimen) on functional and adaptive properties of the muscle. Conclusion: minimal manifestations of destructive changes in muscle fibers of MM recorded in the group of animals with increased physiological activity (swimming before and after surgery), and the most expressed — with reduced one (did not swim).
Chronic low back pain-associated paraspinal muscle dysfunction is not the result of a constitutionally determined «adverse» fiber-type composition / K. Crossman, M. Mahon, P. J. Watson [et al.] // Spine. — 2004. — Vol. 29, № 6. — Р. 628–634.
Body composition, endurance, strength, cross-sectional area, and density of MM Erector Spinae in men with and without low back pain / G. Hultman, M. Nordin, H. Saraste [et al.] // J. Spinal Dis. — 1993. — Vol. 6. — Р. 114–123.
Prives М. G. Human anatomy / М. G. Prives, N. К. Lysenkov, V. S. Bushkevich. — М.: Меdicine, 1985. — 672 р.
Rissanen A. Back muscles and intensive rehabilitation on patients with chronic low back pain. Effects on back muscle structure and function and patient disability: diss. / A. Rissanen. — Jyvaskyla: University of Jyvaskyla, 2004. — 90 p.
Fibre type composition of the human psoas major muscle with regard to the level of its origin / J. Arbanas, G. S. Klasan, M. Nikolic [et al.] // J. Anat. — 2009. — Vol. 215. — P. 636–641, doi: 10.1111/j.1469-7580.2009.01155.x.
Regional myosin heavy chains distribution in selected paraspinal muscles / G. J. Regev, C. W. Kim, B. E. Thacker [et al.] / Spine. — 2010. — Vol. 35 (13). — P. 1265–1270, doi: 10.1097/BRS.0b013e3181bfcd98.
Lumbar muscles: Structure and function / H. Kalimo, J. Rantanen, T. Viljanen [et al.] // Ann. Med. — 1989. — Vol. 21. — P. 353–359.
Dofferhof A. S. M. The stabilising function of the mm. iliocostales and the mm. multifidi during walking / A. S. M. Dofferhof, P. Vink / J. Anat. — 1985. — Vol. 140. — P. 329–336.
The effect of different physical activity levels on muscle fiber size and type distribution of lumbar multifidus. A biopsy study on low back pain patient groups and healthy control subjects / N. Mazis, D. J. Papachristou, P. Tyllanakis [et al.] // Eur. J. Phys. Rehabil. Med. — 2009. — Vol. 45. — P. 459–467.
Demoulin C. Spinal muscle evaluation in healthy individuals and low-beck-pain patients a literature review / C. Demoulin, J. M. Clielaard, M. Vanderthommen // Joint Bone Spine. — 2007. — Vol. 74, № 1. — P. 9–13.
Modeling vertebral fixation using transpedicular constructions on the lumbar spine of rats / V. О. Radchenko, А. G. Skidanov, G. V. Іvanov [et al.] // Orthopedics, Traumatology and Prosthetics. — 2014. — № 3. — P. 86–89, doi: http://dx.doi.org/10.15674/0030-59872014386-89.
Patent 94502 Ukraine. МПК А61В 17/56 (2006.01). Interbody fusion method in experimental animals / Radchenko V. О., Skidanov А. G., Іvanov G. V., Steshenko V. M.; applicant and patent owner SI «Sytenko Institute of Spine and Joints Pathology National Academy of Medical Sciences of Ukraine». — № u201407005; appl. 23.06.2014; edit 10.11.2014, Bulletin № 21.
European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes. Strasbourg, 18.03.1986 [Electron resource: http://conventions.coe.int/Treaty/EN/Treaties/Html/123.htm.
Sarkisov S. D. Microscopy technique / D. S. Sarkisov, U. L. Perova. — Moscov: Medicine, 1996. — 542 p.
Lilli R. Histopathological technique and practical histochemistry / R. Lilli. — M.: Мir, 1969. — 646 p.
Muscle fibre size and type distribution in thoracic and lumbar regions of erector spinae in healthy subjects without low back pain: normal values and sex differences / A. F. Mannion, G. A. Dumas, R. G. Cooper [et al.] // J. Anat. — 1997. — Vol. 190. — P. 505–513.
Histologic and electrophysiological changes of the paraspinal muscle after spinal fusion. An experimental study / Y. Hu, H. B. Leung, W. W. Lu, K. D. K Luk // Spine. — 2008. — Vol. 33, № 13. — Р. 1418–1422, doi: 10.1097/BRS.0b013e3181753bea.
Manual of Pathological Anatomy / Ed. by Т. P. Vinogradova. — М.: Меdgiz, 1962. — Vol. 6. — 518 p.
Copyright (c) 2015 Artem Skidanov, Natalya Ashukina, Zinayda Danishchuk, Inna Batura, Volodymyr Radchenko
This work is licensed under a Creative Commons Attribution 4.0 International License.
The authors retain the right of authorship of their manuscript and pass the journal the right of the first publication of this article, which automatically become available after 6 months from the date of publication under the terms of Creative Commons Attribution License, which allows others to freely distribute the published manuscript with mandatory linking to authors of the original research and the first publication of this one in this journal.
Authors have the right to enter into a separate supplemental agreement on the additional non-exclusive distribution of manuscript in the form in which it was published by the journal (i.e. to put work in electronic storage of an institution or publish as a part of the book) while maintaining the reference to the first publication of the manuscript in this journal.
The editorial policy of the journal allows authors and encourages manuscript accommodation online (i.e. in storage of an institution or on the personal websites) as before submission of the manuscript to the editorial office, and during its editorial processing because it contributes to productive scientific discussion and positively affects the efficiency and dynamics of the published manuscript citation (see The Effect of Open Access).