Structural features multifidus muscle of rats after transpedicular fixation of vertebrae by various conditions of physical activity

Artem Skidanov, Nataliya Ashukina, Zinayda Danishchuk, Inna Batura, Volodymyr Radchenko

Abstract


Multifidus muscles located in the middle layer of paravertebral muscles provide support for the positioning of the spine, its rota­tion and extension movements. Changing in their structure with age, resulting in injuries or degenerative processes lead to their dysfunction. Objective: To investigate the structural features of the multifidus muscle (MM) in rats with different physical activity after the stabilization of the vertebral bodies LІV–LV . Methods: Experiments were performed on 20 laboratory rats (aged 5 months, weight from 430 to 500 g). The animals were divided into 4 groups: swam before and after (I), before (II) and after (III) surgery, did not swim (IV). The bodies of the adjacent vertebrae LІV–LV were fixed with author pedicle construct. After 3 months after surgery with help of histology and histochemistry methods we studied MM structure at three levels – cranially (LІІІ–LІV ), at the level LІV–LV , and caudally (LV–LVІ ) from zone of surgery. Results: such changes in the structure of muscle fibers MM, as swelling, loss of cross striation and polygonal, replacing adipose tissue, and fibrous proliferation were identified. Manifestations of regeneration were associated with non-uniform density of nuclei. In rats of group II and III content of fat tissue was significantly increased, and in IV — fat and connective tissues compared to group I. Histochemical analysis revealed that the amount of type I muscle fibers in animals of group I was larger compared to the rats of group II to 2,7 % (P> 0,05), III — to 5,77 % (P < 0,05), IV — on 11,12 % (P < 0,01), indicating positive influence of motor activity (swimming regimen) on functional and adaptive properties of the muscle. Conclusion: minimal manifestations of destructive changes in muscle fibers of MM recorded in the group of animals with increased physiological activity (swimming before and after surgery), and the most expressed — with reduced one (did not swim).

Keywords


multifidus muscle; histology; lumbar spine; stabilization of the vertebrae; rats

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DOI: https://doi.org/10.15674/0030-59872015285-91

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