Regeneration of long bone after implantation of β-tricalcium phosphate into its defect
Purpose: in the defect of the rats' femoral shaft to investigate the rate of resorption of osteoplastic material сhronOS™, morphological features in adjacent to the site of implantation of the parent bone, quantitative and qualitative characteristics of the bone tissue regenerate and the nature of its interaction with the implant. Methods: The experiment was conducted on 12 white laboratory male rats (8 months, weight (250 ± 10) g). Under ketamine anesthesia in the middle third of the femoral shaft using a portable drill with spherical cutter at low speed with cooling we created defect diameter of 2.5 mm till the medullary canal which was filled with osteoplastic materials сhronOS™ (Synthes, Switzerland) in the shape of a block without any rigid fixation. Fragments of injured bones were examined after 60 and 120 days after implantation of сhronOS™ by methods of light microscopy with morphometry and scanning electron microscopy. Results: Maternal bones at all stages of observations revealed the presence of gaps with typical osteocytes which indicates a high biocompatibility with сhronOS™. In the area of the defect lamellar bone regenerate was detected which is represented by continuous fields with integrated into its structure recrement of osteoplastic material and separate growths of specific round shape with a high content of osteocytes and osteoblasts interconnected with one another. Growths of bone tissue corresponded in size and shape to pores of сhronOS™ which proves its osteoconductive properties. In micropores of сhronOS™ located osteogenic cells indicating their high affinity for osteoplastic materials. The ratio of the area of osteoplastic material and bone tissue on the 60th day of the experiment was (22,55 ± 1,21) and (77,45 ± 3,23) %, and on the 120th — (17,65 ± 1,09) and (82,3 ± 3,35) %, respectively.
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