Clonogenic activity of bone marrow stromal stem cells in various forms of course of idiopathic and dysplastic coxarthrosis

Abstract

Objective: To determine clonogenic activity of bone marrow stromal stem cells (BMSSC) in bones forming the hip joint in patients with various forms of course of idiopathic and dysplastic coxarthrosis (CA). Methods: We cloned BMSSC according to A. J. Friedenstein method. A total of 56 patients with idiopathic and 23 dysplastic CA stage IV were examined. We studied 201 bone marrow samples (140 — idiopathic and 61 — dysplastic CA), 325 cultures of BMSSC were grown. Cancellous bone was taken from three sites: the roof of the acetabulum, femoral head and intertrochanteric area. Course of CA was determined according to the working classification developed. We used also statistical methods. Results: It was found that the higher the course rate of idiopathic or dysplastic CA the lower clonogenic activity of BMSSC in bones forming the hip joint. It was revealed that the state of the connective tissue influences directly to the development of violations in trophic processes in bone tissue of patients with a form of rapid course of CA which is confirmed by the results of X-ray (atrophic type of bone formation according to Bombelli), biochemical (decreasing of content of glycosaminoglycans and protein synthesizing fraction of hydroxyproline in serum) and other studies. Conclusions: There was an inverse relationship between the clonogenic activity of BMSSC in bones forming the hip joint, and the speed (and consequently form) of course for idiopathic and dysplastic CA. There was proved significant (p ≤ 0,05) decreasing in osteogenic potency of BMSSC in patients with a form of rapid course of CA. Revealed features bring closer to understanding the complex of pathogenic mechanisms of idiopathic and dysplastic CA, prove an important role of connective tissue dysplasia in it and provide a differentiated approach to the methods of surgical treatment (use of bone autoplasty, etc.).