THE INFLUENCE OF THE CULTURE OF FIBROBLASTIC CELL ELEMENTS ON THE INDICATORS OF THE METABOLISM OF CONNECTIVE TISSUE IN EXPERIMENTAL ANIMALS

Authors

DOI:

https://doi.org/10.15674/0030-59872024365-69

Keywords:

Сollagenase, hydroxyproline, glycosaminoglycans, fibroblasts, tendon damage

Abstract

The number of patients with degenerative tendon disease affects millions of people both among athletes and the general population, causing significant socio-economic consequences. Despite the availability of various methods of conservative and surgical treatment, more than a third of patients experience constant pain. Objective. To study the indicators of the metabolism of connective tissue in animals with a model of degenerative damage to tendons against the background of the introduction of a culture of fibroblastic cell elements. Methods. Therefore, the development of methods for restoring the structure of tendons using cell cultures, in particular fibroblasts, will allow to optimize the course of reparative processes, reduce the risk of complications during surgical intervention and accelerate healing, and at the molecular level — to improve the structure of collagen fibers. Laboratory studies of biochemical markers of a tendon with a degenerative-dystrophic lesion and against the background of the introduction of cell culture can help in the differential diagnosis of its extracellular matrix. Results. The experimental data obtained by us indicate the presence of differences in the biochemical markers of tendons with degenerative-dystrophic lesions in rats 7, 21, and 45 days after the introduction of culture of fibroblastic cell elements. However, 45 days after the introduction of the culture of fibroblast cell elements, the normalization of metabolic processes in the extracellular matrix of connective tissue occurs, namely, the activity of collagenase and the concentration of protein-bound hydroxyproline approaches normal values. This indicates the predominance of the synthetic phase over the catabolic one in collagen metabolism. Conclusions. In this context, the introduction of culture of fibroblastic cell elements, as an alternative anti-inflammatory method, may provide another potential opportunity in the treatment of chronic degenerative-dystrophic lesions of the Achilles tendon.

Author Biographies

Sadrudin Magomedov, «Institute of Traumatology and Orthopedics of the National Academy of Sciences of Ukraine», Kyiv

MD, Prof.

Yurii Polyachenko, «Institute of Traumatology and Orthopedics of the National Academy of Sciences of Ukraine», Kyiv

MD, Prof.

Оlexandr Kostrub, «Institute of Traumatology and Orthopedics of the National Academy of Sciences of Ukraine», Kyiv

MD, Prof. in Traumatology and Orthopaedics

Roman Blonskyi, «Institute of Traumatology and Orthopedics of the National Academy of Sciences of Ukraine», Kyiv

MD, DSci in Orthopaedics and Traumatology

Ivan Zasadnyuk, «Institute of Traumatology and Orthopedics of the National Academy of Sciences of Ukraine», Kyiv

MD, PhD in Orthopaedics and Traumatology

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How to Cite

Magomedov, S., Polyachenko, Y. ., Kostrub О. ., Blonskyi, R. ., & Zasadnyuk, I. . (2024). THE INFLUENCE OF THE CULTURE OF FIBROBLASTIC CELL ELEMENTS ON THE INDICATORS OF THE METABOLISM OF CONNECTIVE TISSUE IN EXPERIMENTAL ANIMALS. ORTHOPAEDICS TRAUMATOLOGY and PROSTHETICS, (3), 65–69. https://doi.org/10.15674/0030-59872024365-69

Issue

No. 3 (2024)

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ORIGINAL ARTICLES

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Copyright (c) 2024 Садрудін Магомедов, Юрій Поляченко, Олександр Коструб, Роман Блонський, Іван Засаднюк

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