BIOCHEMICAL PARAMETERS OF THE BLOOD OF RATS OF DIFFERENT AGES AFTER FILLING THE METAPHYSEAL DEFECT OF THE FEMUR WITH ALLOGENEIC BONE IMPLANTS WITH LOCAL ADMINISTRATION OF BLOOD PLASMA ENRICHED WITH PLATELETS

Authors

DOI:

https://doi.org/10.15674/0030-59872024357-64

Keywords:

Bone tissue, defect, allograft, biochemistry, regeneration

Abstract

A promising method of regenerative medicine is the saturation of allografts with platelet-rich plasma (PRP). Objective. To evaluate the course of metabolic processes after filling a hole defect in the distal metaphysis of the femur with allogeneic bone implants in conditions of additional local administration of allogeneic PRP. Methods. In the model of a hole defect in white rats with the filling of the defect with an allograft, as well as with additional local stimulation of PRP on the 7th day; on the 3rd and 7th days and on the 1st, 3rd and 7th days in blood serum, the content of glycoproteins (GP), chondroitin sulfates (CST), total protein (TP), calcium (Ca), activity alkaline phosphatase (AlP) and acid phosphatase (AcP). The results. 14 day. In the 3-month rats, under one stimulation, an increase in TP and Ca, a decrease in AcP was observed, with two stimulations there was a 1.11 times smaller GP, 1.13 times larger TP and 1.43 times Ca compared to those in rats without stimulation. During three stimulations, the GP was 1.24 times lower than that in animals without stimulation. In the 12-month rats in comparison with the data of rats without stimulation, 1.15 times higher GP, Ca, AlP activity, and 1.44 times more AcP were noted. 28 days of the 3-month rats for one injection exceeded the data of animals without stimulation by 1.34 times for GP, and were inferior to them by 1.31 times for AcP. In the 12-month rats, compared to these animals without stimulation, with three injections, a 1.19 times greater TP was noted. 90 d. In the 3-month rats for one injection showed 1.24 times less CST with 1.28 times lower AlP compared to data from rats without stimulation. 12-month rats exceeded the data of the group without stimulation by 1.43 times for AlP. Conclusions. In rats with an alloimplant (especially at 12 months), an increase in connective tissue formation markers and a decrease in LF activity were observed. Filling the defect with an alloimplant led to an increase in inflammation indicators and an increase in markers of bone tissue formation.

Author Biographies

Petro Vorontsov, Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of Ukraine, Kharkiv

MD, PhD in Traumatology and Orthopaedics

Frieda Leontyeva, Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of Ukraine, Kharkiv

PhD in Biol. Sci

Vladyslav Tuliakov, Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of Ukraine, Kharkiv

DSci in Pharmacy

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How to Cite

Vorontsov, P. ., Leontyeva, F. ., & Tuliakov, V. . (2024). BIOCHEMICAL PARAMETERS OF THE BLOOD OF RATS OF DIFFERENT AGES AFTER FILLING THE METAPHYSEAL DEFECT OF THE FEMUR WITH ALLOGENEIC BONE IMPLANTS WITH LOCAL ADMINISTRATION OF BLOOD PLASMA ENRICHED WITH PLATELETS. ORTHOPAEDICS TRAUMATOLOGY and PROSTHETICS, (3), 57–64. https://doi.org/10.15674/0030-59872024357-64

Issue

No. 3 (2024)

Section

ORIGINAL ARTICLES

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Copyright (c) 2024 Петро Воронцов, Фріда Леонтьєва, Владислав Туляков

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