CHANGES IN THE CONTENT AND ACTIVITY OF MUSCLE PROTEINS AS A MARKER IN THE CASE OF EXPOSURE TO IONIZING RADIATION IN ANIMALS

Authors

DOI:

https://doi.org/10.15674/0030-59872024349-56

Keywords:

Skeletal and cardiac muscle, ionizing radiation, irradiated descendents, contractile proteins, ATPase activity of actomyosin and myosin, muscle dysfunction, pathogenetic mechanisms

Abstract

Objective. To find out the specifics of muscle tissue contraction under the conditions of exposure to low doses of ionizing radiation in parents and their irradiated offspring by evaluating changes in the content of contractile proteins and ATPase activity of skeletal and cardiac muscles. Methods. Experimental studies were conducted on 50 rats. Animals were randomized as follows: group 1 — intact sexually mature rats; 2 — sexually mature animals irradiated with a dose of 1.0 Gy; group 3 — monthold rats obtained from intact individuals; group 4 — month-old rat pups obtained from parents irradiated with a dose of 1.0 Gy; group 5 — month-old rats obtained from animals irradiated with a dose of 1.0 Gy, which were irradiated at the same dose. The results. Muscle dysfunction in irradiated offspring and parents is manifested by a decrease in the content of contractile proteins, functional dysfunction of the actomyosin bridge, and a decrease in the ATPase activity of contractile proteins. Marked muscle dysfunctions during the post-radiation time period may be the reason for the formation of orthopedic pathology in a significant contingent of irradiated persons. Conclusions. The expression of muscle dysfunction in the offspring of irradiated animals, which were also exposed to ionizing radiation, is greater than the corresponding processes in their irradiated parents, which indicates the mediation of muscle dysfunction in the second generation of irradiated animals by epigenetic mechanisms. Marked muscle dysfunctions during the post-radiation time period may be the reason for the formation of orthopedic pathology in a significant contingent of irradiated persons

Author Biographies

Igor Lurin, National Academy of Medical Sciences of Ukraine, Kyiv

MD, Prof.

Igor Khomenko, National Academy of Medical Sciences of Ukraine, Kyiv

MD, Prof.

Gennadiy Stepanov, Odesa National Medical University. Ukraine

MD, D.Sci.

Serhii Tertyshnyi, Odesa National Medical University. Ukraine, Military Medical Clinical Center of the Southern Region of the Command of the Medical Forces of the Armed Forces of Ukraine, Odesa

MD, D.Sci.

Rooslan Vastyanov, Odesa National Medical University. Ukraine

MD, Prof.

Oksana Tiron, Odesa National Medical University. Ukraine

MD, PhD:

References

  1. Moroz, V. M., Shandra, O. A., Vastyanov, R. S., & Omelchenko, O. D. (2016). Physiology. Vinnytsia: Nova Knyha. (in Ukrainian)
  2. Rashid, S, & Riaz, A, (2023). Ultrasonication induced alterations in physicochemical and functional properties of myosin. Protein & Peptide Letters,30(3), 221-232. https://doi.org/doі: 10.2174/0929866530666230124093804
  3. Golkа, H. G., Bur'yanov, O. A., & Klimovytskyi, V. G. (2019). Traumatology and orthopedics: a textbook for students of higher medical educational institutions. Vinnytsia: Nova Kniga. (in Ukrainian).
  4. Azarov, S. I, Yeremenko, S. A, Sydorenko. V. L, Biloshitsky. M. V, Vlasenko, E. A, & Pruskyi, A. V, (2019). Technogenic and ecological consequences of the Chernobyl disaster. Kyiv: MPBP "Gordon".(in Ukrainian).
  5. Tsymbalyuk, V. I., (2021). Atlas of combat surgical trauma (experience of anti-terrorist operation/operation of joint forces). Kharkiv: Collegium. (in Ukrainian).
  6. Tsymbalyuk, V. I., (2020). Gunshot wounds of soft tissues (experience of anti-terrorist operation/operation of joint forces). Kharkiv: Collegium. (in Ukrainian).
  7. Zarutskyi, Y. L., & Bilyi, V. Y., (2018). Military field surgery: practice. and teach manual for the troops doctors and doctors of the healthcare system of Ukraine. Kyiv: Phoenix. (in Ukrainian).
  8. Salyutin, R. V, Kashtalyan, M. A, Lurin, I. A, Khomenko, I. P, Negoduyko, V. V, Mykhailosov, R. M, Humenyuk, K. V, & Tertyshny, S. V. (2021) Atlas of combat surgical trauma (counterterrorism/joint force operation experience). Kharkiv: Collegium.
  9. Xu, P., Yi, Y., Luo, Y., Liu, Z., Xu, Y., Cai, J., Zeng, Z., & Liu, A. (2021). Radiation‑induced dysfunction of energy metabolism in the heart results in the fibrosis of cardiac tissues. Molecular Medicine Reports, 24(6). https://doi.org/10.3892/mmr.2021.12482
  10. Bondar, O. I, Vashchenko, V. M, Azarov, S. I, Sydorenko, V. L, Loza, E. A, & Korduba, I. B., (2019). Chernobyl in the fourth decade. Kyiv: Enterprise "NAIR". (in Ukrainian).
  11. Bahar, M. R., Tekin, S., Beytur, A., Onalan, E. E., Ozyalin, F., Colak, C., & Sandal, S. (2023). Effects of intracerebroventricular MOTS-C infusion on thyroid hormones and uncoupling proteins. Biologia Futura, 74(1–2), 159–170. https://doi.org/10.1007/s42977-023-00163-6
  12. Mansson, A., & Rassier, D. E. (2022). Insights into muscle contraction derived from the effects of small-molecular actomyosin-modulating compounds. International Journal of Molecular Sciences, 23(20), 12084. https://doi.org/10.3390/ijms232012084
  13. Baynes, J. W., & Dominiczak, M. H. (2023). Medical biochemistry: Edited by John W. Baynes, Marek H. Dominiczak.
  14. Duno-Miranda, S., Nelson, S. R., Rasicci, D. V., Bodt, S. L., Cirilo, J. A., Vang, D., Sivaramakrishnan, S., Yengo, C. M., & Warshaw, D. M. (2023). Tail length and E525K dilated
  15. ISSN 0030-5987. Ортопедія, травматологія та протезування. 2024. № 3 cardiomyopathy mutant alter human β-cardiac myosin superrelaxed state. https://doi.org/10.1101/2023.12.07.570656
  16. Cakar, M. M., Milсiс, N., Andreadaki, T., Charnock, S., Fessner, W., & Blazeviс, Z. F. (2024). Kinetic characterization of two neuraminic acid synthases and evaluation of their application potential. Applied Microbiology and Biotechnology, 108(1). https://doi.org/10.1007/s00253-024-13277-1
  17. Lapovets, L. E, Lebe, G. B, & Yastremska, O. O., (2019). Clinical laboratory diagnostics. Kyiv: All-Ukrainian specialized publishing house "Medicine".(in Ukrainian).
  18. Stepanov, G. F., & Vastyanov, R. S. (2023). Involvement of intramuscular pathology at the level of the actomyosin Junction into the pathogenetic mechanisms of muscle dysfunctionsin the descendents of irradiated rats. World of Medicine and Biology, 19(85), 230. https://doi.org/10.26724/2079-8334-2023-3-85-230-23
  19. Attia, A. M., Aboulthana, W. M., Hassan, G. M., & Aboelezz, E. (2019). Assessment of absorbed dose of gamma rays using the simultaneous determination of inactive hemoglobin derivatives as a biological dosimeter. Radiation and Environmental Biophysics, 59(1), 131–144. https://doi.org/10.1007/s00411-019-00821-1
  20. Stepanov, G. F., & Vastyanov, R. S. (2023). The peculiarities of low-dose ionizing radiation influence on muscles metabolism in experimental animals. World of Medicine and Biology, 19(84), 233. https://doi.org/10.26724/2079-8334-2023-2-84-233-238
  21. Vastyanov, R. S, Stoyanov, A. N, & Bakumenko, I. K., (2015). Systemic pathological disintegration in chronic cerebral ischemia. Experimental and clinical aspects. Saarbrucken : LAP Lambert Academic Publishing. (in russian).

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How to Cite

Lurin, I. ., Khomenko, I. ., Stepanov, G. ., Tertyshnyi, S. ., Vastyanov, R. ., & Tiron, O. . (2024). CHANGES IN THE CONTENT AND ACTIVITY OF MUSCLE PROTEINS AS A MARKER IN THE CASE OF EXPOSURE TO IONIZING RADIATION IN ANIMALS. ORTHOPAEDICS TRAUMATOLOGY and PROSTHETICS, (3), 49–56. https://doi.org/10.15674/0030-59872024349-56

Issue

No. 3 (2024)

Section

ORIGINAL ARTICLES

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Copyright (c) 2024 Ігор Лурін, Ігор Хоменко, Геннадій Степанов, Сергій Тертишний, Руслан Вастьянов, Оксана Тірон

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