BIOCHEMICAL INDICATORS OF BLOOD SERUM OF RATS OF DIFFERENT AGES AFTER FILLING THE DEFECT IN THE METAPHYSIS OF THE FEMUR WITH ALLOGENEIC BONE IMPLANTS

Authors

  • Petro Vorontsov Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of Ukraine, Kharkiv, Ukraine https://orcid.org/0000-0002-5758-7223
  • Frieda Leontyeva Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of Ukraine, Kharkiv, Ukraine
  • Vladyslav Tuliakov Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of Ukraine, Kharkiv, Ukraine

DOI:

https://doi.org/10.15674/0030-59872023134-40

Keywords:

bone defect, experimental modelling, regeneration, biochemistry, connective tissue

Abstract

Bone defects that do not heal on their own are a significant problem in orthopaedic and trauma surgery. One of the approaches to its solution is the use of bone alloimplants (AloI). Objective. On the basis of the analysis of biochemical indicators of the metabolism of connective tissue in the blood serum of laboratory rats, the course of metabolic processes after the filling of the defect in the metaphysis of the AloI femur was evaluated. Methods. A model of creating a transcortical defect of critical size (diameter 3 mm, depth 3 mm) in the metaphysis of the femur of 3- and 6-month-old rats was used. In animals of groups I (n = 15, age 3 months) and III (n = 15,12 months) the defects were left unfilled, II (n = 15, 3 months) and IV (n = 15, 12 months) — filled with structural AloI. After 14, 28 and 90 days, the content of glycoproteins, total chondroitin sulphates (CS), protein and calcium, activity of alkaline and acid phosphatases in blood serum was investigated. Results. The introduction of AloI leads to an increase in the content of glycoproteins for all periods
of observation in rats of both age groups. 14 days after implantation in 12-month-old rats, compared to 3-month-old rats, a 1.30 times higher level of CS in blood serum was determined (p = 0.008), which is due to their higher content in the area of connective tissue implantation; the activity of alkaline phosphatase decreased by 1.80 times p = 0.016) and acid phosphatase by 1.50 times (p = 0.018), which indicates a delay in the formation and reorganization of bone tissue. However, the level of CS under the conditions of the establishment of AloI on the 90th day was lower compared to the corresponding
groups without plasticity of the defect: in 3-month-old rats by 1.44 times ( p = 0.008), in 12-month-old rats by 1.52 times (p = 0.008). Conclusions. According to the indicators of biochemical markers of connective tissue metabolism, the use of AloI for plasticity of defects of a critical size in the metaphysis of the femur of rats leads to the activation of bone regeneration with a greater manifestation in younger recipients compared to groups with an unfilled
defect.

Author Biographies

Petro Vorontsov, Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of Ukraine, Kharkiv

MD, PhD in Traumatology and Ortho

Frieda Leontyeva, Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of Ukraine, Kharkiv

PhD in Biol. Sci

Vladyslav Tuliakov, Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of Ukraine, Kharkiv

DSci in Pharmacy

References

  1. Huang, Q., Xu, Y. B., Ren, C., Li, M., Zhang, C. C., Liu, L., Wang, Q., Lu, Y., Lin, H., Li, Z., Xue, H. Z., Zhang, K., & Ma, T. (2022). Bone transport combined with bone graft and internal fixation versus simple bone transport in the treatment of large bone defects of lower limbs after trauma. BMC Musculoskeletal Disorders, 23(1). https://doi.org/10.1186/s12891-022-05115-0
  2. Cohen, J. D., Kanim, L. E., Tronits, A. J., & Bae, H. W. (2021). Allografts and Spinal Fusion. International Journal of Spine Surgery, 15(s1), 68–93. https://doi.org/10.14444/8056
  3. Petersen, L., Baas, J., Sørensen, M., Bechtold, J. E., Søballe, K. & Barckman, J. (2022). Accelerated bone growth, but impaired implant fixation in al¬lograft bone mixed with nano-hydroxyapatite - an experimental study in 12 canines. Journal of experimental orthopaedics, 9 (1), 35. doi: 10.1186/s40634-022-00465-z.
  4. Nauth, A., Schemitsch, E., Norris, B., Nollin, Z., & Watson, J. T. (2018). Critical-Size Bone Defects. Journal of Orthopaedic Trauma, 32, S7-S11. https://doi.org/10.1097/bot.0000000000001115
  5. Haugen, H. J., Lyngstadaas, S. P., Rossi, F., & Perale, G. (2019). Bone grafts: which is the ideal biomaterial? Journal of Clinical Periodontology, 46, 92–102. https://doi.org/10.1111/jcpe.13058
  6. Baldwin, P., Li, D. J., Auston, D. A., Mir, H. S., Yoon, R. S., & Koval, K. J. (2019). Autograft, Allograft, and Bone Graft Substitutes. Journal of Orthopaedic Trauma, 33(4), 203–213. https://doi.org/10.1097/bot.0000000000001420
  7. Archunan, M. W., & Petronis, S. (2021). Bone Grafts in Trauma and Orthopaedics. Cureus. https://doi.org/10.7759/cureus.17705
  8. Feltri, P., Solaro, L., Di Martino, A., Candrian, C., Errani, C., & Filardo, G. (2022). Union, complication, reintervention and failure rates of surgical techniques for large diaphyseal defects: a systematic review and meta-analysis. Scientific Reports, 12(1). https://doi.org/10.1038/s41598-022-12140-5
  9. Lei, P.-f., Hu, R.-y., & Hu, Y.-h. (2019). Bone Defects in Revision Total Knee Arthroplasty and Management. Orthopaedic Surgery, 11(1), 15–24. https://doi.org/10.1111/os.12425
  10. European Convention for the protection of vertebrate animals used for research and other scientific purposes. Strasbourg, 18 March 1986: official translation. Verkhovna Rada of Ukraine. (In Ukrainian). URL: http://zakon.rada.gov.ua/cgi-bin/laws/ main.cgi?nreg=994_137. 21.
  11. On protection of animals from cruel treatment: Law of Ukraine №3447-IV of February 21, 2006. The Verkhovna Rada ofUkraine. (In Ukrainian). URL: http://zakon.rada.gov.ua/cgi-bin/laws/ main.cgi?nreg=3447-15.
  12. Ashukina, N., Maltseva, V., Vorontsov, P., Danyshchuk, Z., Nikolchenko, O., & Korzh, M. (2022). Histological evaluation of the incorporation and remodeling of structural allografts in critical size metaphyseal femur defects in rats of different ages. Romanian Journal of Morphology and Embryology, 63(2), 349–356. https://doi.org/10.47162/rjme.63.2.06
  13. Kamyshnikov, V. S. (2003). Clinical and biochemical laboratory di¬agnostics: reference book: in 2 volumes [Kliniko-biokhimicheskaya laboratornaya diagnostika: spravochnik: v 2 t.] (2nd ed.). Minsk : Interpressservice. (in russian).
  14. Morozenko, D. V., Leontieva, F. S. (2016). Research methods markers of connective tissue metabolism in modern clinical and experimental medicine [Metody doslidzhennya markeriv metabolizmu spoluchnoyi tkanyny u klinichniy ta eksperymentalʹniy medytsyni]. Molodyy vchenyy, № 2 (29), 168–172. (in Ukrainian).
  15. Lang, T. A., Sesik, M. M. (2011). How to describe statistics in medicine. A guide for authors, editors and reviewers [Kak opisyvat' statistiku v meditsine. Rukovodstvo dlya avtorov, redaktorov i retsenzentov]. Moscow : Practical Medicine. (in russian)
  16. van der Donk, S., Buma, P., Slooff, T. J. J. H., Gardeniers, J. W. M., & Schreurs, B. W. (2002). Incorporation of Morselized Bone Grafts: A Study of 24 Acetabular Biopsy Specimens. Clinical Orthopaedics and Related Research, 396, 131–141. https://doi.org/10.1097/00003086-200203000-00022
  17. Hooten, J. P., Engh, C. A., Heekin, R. D., & Vinh, T. N. (1996). Structural bulk allografts in acetabular reconstruction. The Journal of Bone and Joint Surgery. British volume, 78-B(2), 270–275. https://doi.org/10.1302/0301-620x.78b2.0780270

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How to Cite

Vorontsov, P. ., Leontyeva, F. ., & Tuliakov, V. (2023). BIOCHEMICAL INDICATORS OF BLOOD SERUM OF RATS OF DIFFERENT AGES AFTER FILLING THE DEFECT IN THE METAPHYSIS OF THE FEMUR WITH ALLOGENEIC BONE IMPLANTS. ORTHOPAEDICS TRAUMATOLOGY and PROSTHETICS, (1), 34–40. https://doi.org/10.15674/0030-59872023134-40

Issue

No. 1 (2023)

Section

ORIGINAL ARTICLES

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