Modeling of fixation with using of transpedicular constructs in the lumbar spine of the rats

Volodymyr Radchenko, Artem Skidanov, Gennadiy Ivanov, Nataliya Ashukina, Petr Levytskyi

Abstract


Despite on significant progress in the development of vertebrology the problem of treatment of degenerative diseases of the spine remains relevant due to the significant amount of people suffering from back pain. One of the approaches to the study of the etiopathogenesis of degenerative diseases of the spine might be conducting experimental studies with using of histological methods to develop new ways for prevention and treatment of this disease. Objective: To develop an experimental model of transpedicular fixation of the lumbar spine in the rats. Methods: For reproducing the experimental model lab rats aged 5 months, weighing from 430 to 500 g of a population of experimental biological clinic of the SI «Sytenko Institute for Spine and Joints Pathology of the National Academy of Medical Sciences of Ukraine». Surgeries in animals were performed aseptically under general anesthesia (chlorpromazine — 10 mg/kg, ketamine — 50 mg/kg). After cleaning of the operating field with a solution of iodine and surgical approach we exposed posterior parts of two adjacent vertebrae. Further we modeled violation of innervation for paravertebral muscles which usually occurs in a compression-radicular syndromes through the destruction of the posterior branch of the spinal nerve. We performed bone autoplasty. Results: For the study of the morphological features of the paravertebral muscles in cases of fixation of adjacent vertebral bodies there were designed an appropriate construct which was implemented to the experimental rats and tool kit for it. Radiography done immediately after surgery and at 3 months after it discovered the stability of fixation. Conclusion: The developed construct for fixation of the vertebral bodies of the rats allows modeling surgeries for posterior transpedicular stabilization, provides possibilities for studying factors affecting quality of fusion in particular the state of paravertebral muscles.


Keywords


experiment; rats; transpedicular construct; lumbar spine

References


Korzh N. A. Degenerative diseases of spine and their structural functional classification / N. A. Korzh, A. I. Prodan, A. E. Barysh // Ukrainian neurosurgical journal. — 2004. — № 3. — Р. 71–80.

Chronic low back pain-associated paraspinal muscle dysfunction is not the result of a constitutionally determined “adverse” fiber-type composition / K. Crossman, M. Mahon, P. J. Watson [et al.] // Spine. — 2004. — Vol. 9, № 6. — Р. 628–634.

Body composition, endurance, strength, cross-sectional area, and density of mm erector spinae in men with and without low back pain / G. Hultman, M. Nordin, H. Saraste [et al.] // J. Spinal Dis. — 1993. — Vol. 6. — Р. 114–123.

Malyshkina S. V. Experimental modeling in scientific researches of Sytenko Institute of spine and joint pathology / S. V. Malyshkina // Orthopaedy, traumatology and prosthetics. — 2007. — № 4. — Р. 5–16.

BMP-2 induced early bone formation in spine spondylolysis using rat ovariectomy osteoporosis model / S. B. Park, S. H. Park, N. H. Kim, C. K. Chung // Spine J. — 2013. — Vol. 13 (10). — P. 1273–1280. doi: 10.1016/j.spinee.2013.06.010.

Stem cells from human fat as cellular delivery vehicles in an athymic rat posterolateral spine fusion model / W. K. Hsu, J. C. Wang, N. Q. Liu [et al.] // J. Bone Joint Surg. — 2008. — Vol. 90-A (5). — P. 1043–1052. doi: 10.2106/JBJS.G.00292.

Front fusion in experiment / I. P. Ardashev, V. T. Podorozhnaia, I. A. Kirilova [et al.] // Spine surgery. — 2008. — № 1. — Р. 66–73.

Hvysiuk N. I. Instability of lumbar spine: autoreport dis. ... doctor med. science/ N. I. Hvysiuk. — Kiev, 1977. — 32 p.

Petrenko D. E. Rationale for interbody fusion model in rats / D. E. Petrenko, G. V. Ivanov, A. A. Mezencev // Manual of Karazin university. Sequel Medicine… — 2012. — № 998 (23). — p. 5–10.

European Convention for the Protection of Vertebrate Animals that are used for researches and other scientific purposes. Strasburg, 18 September 1986: official translation [Electronic resource ] / Verkhovna Rada of Ukraine. — Official web-site. — (International document of European council ). — Mode of access to the document: http://zakon.rada.gov.ua/cgi-in/laws/main.cgi?nreg=994_137.

Animal protection from cruelty: Law of Ukraine № 3447 from 21.02.2006 [Electronic resource]/ Verkhovna Rada of Ukraine. — Official web-site. — Mode of access to the document: http://zakon.rada.gov.ua/cgi-bin/laws/main.cgi?nreg=3447-15.

Active therapy for chronic low back pain: Part 1. Effects on back muscle activation, fatigability, and strength / A. F. Mannion, S. Taimela, M. Muntener [et al.] // Spine. — 2001. — № 26. — P. 897–908.

Evaluation of specific stabilizing exercise in the treatment of chronic low back pain with radiologic diagnosis of spondylolysis or spondylolisthesis / P. B. O’Sullivan, G. D. Phyty, L. T. Twomey, G. T. Allison // Spine. — 1997. — № 22. — P. 2959–2967.




DOI: https://doi.org/10.15674/0030-59872014386-89

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