Restructuring of bone tissue under filling bone cavities with carbon synthetic biomaterial

Svitlana Malyshkina, Chzhou Lu, Ninel Dedukh


Experience of research and using of substitute materials in reconstructive surgery of the skeleton shows that «ideal» substitute for natural bone still was not created. So far as the problem of finding biomaterials determined by high requirements for them it does not lose its relevance. Objective: To examine the restructuring of bone in the area of implantation of synthetic dense carbon biomaterial. Methods: In transkortikal hole femoral defects in 30 white laboratory rats dense carbon biomaterial was implanted. For assessment of osteoreparation we used histological, morphometric and electron microscopic methods. After decalcification of bone fragments biomaterial was partially removed out of defect for manufacturing of histological sections (7–9 microns) which was stained with Veyhert hematoxylin and eosin. Histological analysis and photographing of material was performed using a microscope «Axiostar Plus», and ultrastructural analysis — with transmission electron microscope EMV-100BR. Results: It was revealed that under conditions of filling of modeled cavities in the bone testing biomaterial does not cause any inflammatory reaction in studied terms of observation (3–45th day) and does not lead to progression of destructive lesions in the surrounding bone. Since early periods (7th day) it was detected fibroreticular tissue formation of osteogenic nature and newly developed bone tissue on the perimeter of implant cavity, as well as in areas replaced with destructive zone of maternal bone formed in the course of the defect creation. On the 45th day nearly the entire perimeter of the cavity with carbon biomaterial residues newly formed bone in the form of bone trabeculae of lamellar structure located. Conclusion: together with other synthetic implant materials investigated carbon biomaterial can be used for plastic of bone defects of «critical» size.


experiment; rats; bone defect; implantation; carbon biomaterial


Ceramic plastic in orthopedics and traumatology / [A. A. Korzh, G. H. Gruntovsky, N. A. Korzh, V. T. Mykhailo]. – Lviv: «Svit», 1992. – 111 p.

Implant materials and osteogenesis. The role of biological fixation and osseointegration in bone reconstruction / N. A. Korzh, L. A. Kladchenko, S. V. Malyshkina, I. B. Timchenko // Orthopedics, Traumatology and Prosthetics. – 2005. – № 4. – P. 118–127.

Fabrication and evaluation of porous beta-tricalcium phosphate/hydroxyapatite (60/40) composite as a bone graft extender using rat calvarial bone defect model / J. H. Lee, M. Y. Ryu, H.-R. Baek [et al.] // The Scientific World Journal. — 2013. — Vol. 2013. — Р. 121–130.

Effectiveness of synthetic hydroxyapatite versus Persian Gulf coral in an animal model of long bone defect reconstruction / A. Meimandi Parizi, A. Oryan, Z. Shafiei-Sarvestani, A. Bigham-Sadegh // J. Orthopaedics and Traumatology. — 2013. — Vol. 14, Issue 4. — P. 259–268.

Abdollahi S. Surface transformations of Bioglass 45S5 during scaffold synthesis for bone tissue engineering / S. Abdollahi, A. C. Ma, M. Cerruti // Langmuir. — 2013. — Vol. 29, № 5. — P. 1466–1474.

Eglin D. Degradable polymeric materials for osteosynthesis: tutorial / D. Eglin, M. Alini // Eur. Cell Mater. — 2008. — Vol. 16. — P. 80–91.

On the in vitro and in vivo degradation performance and biological response of new biodegradable Mg-Y-Zn alloys / A. C. Hanzi, I. Gerber, M. Schinhammer [et al.] // Acta Biomater. — 2010. — Vol. 6, № 5. — P. 1824–1833.

Eyesan S. U. Bone cement in the management of cystic tumour defects of bone at National Orthopaedic Hospital, Igbobi, Lagos / S. U. Eyesan, O. A. Ugwoegbulem, D. C. Obalum // Niger. J. Clin. Pract. — 2009. — Vol. 12, № 4. — P. 367–370.

Carbon nanotubes in nanocomposites and hybrids with hydroxyapatite for bone replacements [on-line] / U. S. Shin, I.-K. Yoon, G.-S. Lee [et al.] // J. Tissue Eng. — 2011. — Access mode:

Porous calcium phosphate ceramic granules and their behaviour in differently loaded areas of skeleton / Z. Zyman, V. Glushko, N. Dedukh [et al.] // J. Mater. Sci. Mater. Med. — 2008. — Vol. 19 (5). — P. 2197–2205.

Albrektsson T. Osteoinduction, osteoconduction and osseointegration / T. Albrektsson, C. Johansson // Eur. Spine J. — 2001. — № 10. — P. 96–101.

Effect of hydroxyapatite on bone integration in a rabbit tibial defect model / M.-J. Lee, S.-K. Sohn, K.-T. Kim [et al.] // Clin. Orthop. Surg. — 2010. — Vol. 2, № 2. — P. 90–97.

Effects of hydroxyapatite on bone graft resorption in an experimental model of maxillary alveolar arch defects / O. Pilanci, C. Cinar, S. V. Kuvat [et al.] // Arch. Clin. Exp. Surg. — 2013. — Vol. 2, № 3. — P. 170–175.

Rolik A. V. Applications carbon implants in traumatology and orthopedics / A. V. Rolik, S. D. Shevchenko, E. J. Pankow: аbstracts regional scientific-practical conference dedicated to the 80th anniversary of HNIIOT them M. I. Sytenko. — Kharkiv, 1987 — P. 83-85.

Shevchenko S. D. Replacement parietal diaphyseal long bone defects by carbon implants in the experiment / S. D. Shevchenko, A. V. Rolik / Orthopedics, Traumatology and Prosthetics. — 1987. — № 7. — 38–39.

Morphological features of bone regeneration in implant carbon material in the experiment / O. A. Tyazhelov, N. O. Ashukina, G. V. Ivanov [et al.] // Ukrainian Medical Almanac. — 2005. — № 2. — P. 142–144.

European Convention for the Protection of Vertebrate Animals used for experimental and other scientific purposes. Strasbourg, 18 March 1986: official translation [electronic resource] / Parliament of Ukraine. — Off. website. — (International Council of Europe documents). — Access to document:

Evaluation of bone regeneration using the rat critical size calvarial defect / P. P. Spicer, J. D. Kretlow, S. Young [et al.] // Nat Protoc. — 2012. — Vol. 7, № 10. — P. 1918–1929.

Sarkisov D. S. Microscopical technique / D. S. Sarkisov, L. Yu. Perov. — M.: Medicine, 1996. — 542 p.

Weekly B. Electron microscopy for beginners / B. Weekley. — M.: Mir, 1975. — 324 p.

Reparative bone regeneration around titanium implants after exposure to low-intensity pulsed ultrasound / S. V. Malyshkina, V. I. Makolinets, I. V. Vyshniakova [et al.] // Taurian Medical and Biology Vestnik. — 2013. — Vol 16, № 1, Part 1. — P. 147–151.



  • There are currently no refbacks.

Copyright (c) 2014 Svitlana Malyshkina, Chzhou Lu, Ninel Diedukh

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.