Effect of pulsed ultrasound on osteoreparation progress in the area of implantation of titanium samples (experimental study)

Svitlana Malyshkina, Vasyl Makolinets, Olga Nikolchenko, Iryna Vishnyakova, Tamara Grashchenkova

Abstract


Purpose: to study osteoreparative process in experiment on rats in region of implantation of titanium samples after exposure of pulsed ultrasound with oscillations' intensity of 0.4 and 0.7 W/cm2 applied since the third day after surgery. Materials: fragments of femurs with titanium (BT-16) implants of 57 six-month rat became as a material of study. We used morphological and morphometric methods of investigation. We determined area of the newly formed tissue (granulation, fibroreticular and bone) around the implants, active osteoblastic surface (length of bone trabeculae occupied by active osteoblasts), the index of osseointegration (the length of contact surface of the implant with the bone tissue), the thickness of the connective tissue capsule around the perimeter of implant removed. Results: it was found that the pulsed ultrasound accelerates restructuration of granulation tissue to fibroreticular one with its differentiation in osteogenic direction. Presence of significant osteoblasticdifferon cell density in this tissue also indicates to this fact. Ultrasound with oscillations' intensity of 0.4 and 0.7 W/cm2 stimulates bone formation in area of implantation which confirmed by data of active osteoblastic surface, area of newly formed bone tissue, and index of osteointegration which were larger in animals of the experimental groups than in control ones in all terms of the research. Conclusions: more pronounced positive effect of ultrasound on osteoreparation was in the case of the oscillations' intensity of 0.4 W/cm2 which is confirmed by data of area of newly formed bone and osseointegration index obtained at 14, 30 and 45 day of study, and which is veraciously higher than in the case of using of ultrasound intensity of 0.7 W/cm2. Results of the study will be used for substantiation of the development of indications and methods of application of ultrasound in patients after reconstructive surgery with using of fixing devices.


Keywords


low-intensity pulsed ultrasound; titanium implant; femur of the rat; histological investigation

References


Avtandilov G. G. Medical morphometry / G. G. Avtandilov. – М.: Meditsina, 1990. – 381 р.

Dedukh N. V. Bone osseointegration of titanium implants / N. V. Dedukh, S. V. Malyshkina // Orthopaedics, Traumatology and Prosthetics. – 2010. – № 1. – Р. 45–49.

European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes. Strasbourg, 18.III.1986 [electronic resource] – Access mode: http://conventions.coe.int/treaty/en/treaties/html/123.htm

Malyshkina S.V. Effect of low-intensity pulsed ultrasound on the osseointegration of implants (literature review) / S.V. Malyshkina, V.I. Makolinets, I.V. Vishnyakova // Orthopaedics, Traumatology and Prosthetics. – 2012. – № 2. – Р. 122-130.

Microscopic technique. Guide / D.S. Sarkisov, Yu.L. Perov. – М.: Meditsina, 1996. – 544 р.

Shymon V.М. Coatings on titanium implants and osseointegration / V.М. Shymon, S.V. Malyshkina, N.V. Dedukh // Ukrainian Medical Almanac. – 2010. – № 5. – Р. 239-244.

Della Rocca G. J. The science of ultrasound therapy for fracture healing / G. J. Della Rocca // Indian. J. Orthop. — 2009. — Vol. 43, № 2. — Р. 121–126.

Dijkman B. G. Low-intensity pulsed ultrasound: Nonunions / B. G. Dijkman, S. Sprague, M. Bhandari // Indian J. Orthop. — 2009. — Vol. 43, № 2. — Р. 141–148.

Effects of different therapeutic ultrasound intensities on fracture healing in rats / C.-H. Fung, W.-H. Cheung, N. M. Pounder [et al.] // Ultrasound Med. Biol. — 2012. — Vol. 38, № 5. — P. 745–752.

Effects of low intensity pulsed ultrasound on healing of denervated tibial fracture in the rabbit / S. H. Sadraie, Gh. R. Kaka, M. Mofid [et al.] // Iran. Red Crescent Med. J. — 2011. — Vol. 13, № 1. — P. 34–41.

Effects of near-field ultrasound stimulation on new bone formation and osseointegration of dental titanium implants in vitro and in vivo / S. K. Hsu, W. T. Huang, B. S. Liu [et al.] // Ultrasound Med Biol. — 2011. — Vol. 37, № 3. — P. 403–416.

Low-intensity pulsed ultrasound accelerates fracture healing by stimulation of recruitment of both local and circulating osteogenic progenitors / K. Kumagai, R. Takeuchi, H. Ishikawa [et al.] // J. Orthop. Res. — 2012. — Vol. 30, № 9. — P. 1516–1521.

Low-intensity pulsed ultrasound for bone healing: an overview / K. N. Malizos, M. E. Hantes, V. Protopappas, A. Papachristos // Injury Int. Care Injured. — 2006. — Vol. 37S. — P. 56–62.

The evidence of low-intensity pulsed ultrasound for in vitro, animal and human fracture healing / M. P. de Albornoz, A. Khanna, U. G. Longo [et al.] // Br. Med. Bull. — 2011. — Vol. 100. — P. 39–57.

Ultrasound for fracture healing: current evidence / Y. Watanabe, T. Matsushita, M. Bhandari [et al.] // J. Orthop. Trauma. — 2010. — Suppl. 1. — P. 56–61.




DOI: https://doi.org/10.15674/0030-59872014316-23

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Copyright (c) 2014 Svitlana Malyshkina, Vasyl Makolinets, Olga Nikolchenko, Irina Vyshnyakova, Tamara Grashchenkova

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