Modelling of degenerative changes in paravertebral muscles for studying of its influence on spine diseases

Authors

  • Volodymyr Radchenko Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of Ukraine, Kharkiv, Ukraine https://orcid.org/0000-0001-5949-0882
  • Frieda Leontyeva Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of Ukraine, Kharkiv, Ukraine
  • Vladyslav Tuliakov Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of Ukraine, Kharkiv, Ukraine
  • Mykyta Skidanov Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of Ukraine, Kharkiv, Ukraine
  • Artem Skidanov Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of Ukraine, Kharkiv, Ukraine https://orcid.org/0000-0002-6954-9080
  • Olga Nikolchenko Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of Ukraine, Kharkiv, Ukraine https://orcid.org/0000-0001-9808-9485

DOI:

https://doi.org/10.15674/0030-59872021162-68

Keywords:

Muscle, disorders, degeneration, spine, modeling, hyperlipidemia, ligation, biochemistry

Abstract

An important component of the development of degenerative changes in the spine is damage and disruption of the vital acti­vity of the paravertebral muscles. Objective. Based on the analysis of biochemical parameters of laboratory rats serum we evaluated the suitability of the studied models of dystrophic muscle tissue lesions for further study of the development of degenerative-dystrophic disorders in the spinal motor segments. Methods. Simulated: group I (5 female rats) — alimentary obesity by keeping for 3 months. on a high-calorie diet (hyperlipidemic diet); group II (5) — ischemia by ligation for 45 days of large back rectus muscles the with suture material that is not absorbed. Control — 5 intact animals of the same age and sex, which were kept on a standard diet. Serum levels of glycoproteins, haptoglobin, total chondroitin sulfates (CHS), glucose, cholesterol, low-density lipoproteins, triglycerides, total lipids, activity of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline and acid phosphatases, creatine phosphokinase were defined, thymol test was determined. Parameters were processed by the Fisher–Student method. Results. In the I group of rats, the content of glycoproteins, total cholesterol and lipids, low-density lipoproteins, triglycerides, glucose, CHS, ALT and AST activity, thymol test va­lues were increased and the level of creatine phosphokinase activi­ty was decreased. In animals of group II, an increase in serum activity of creatine phosphokinase, glycoproteins and CHS was recorded. Conclusions. Changes in the serum biochemical parameters of white rats recorded on a hyperlipidemic diet indicate the development of fatty degeneration, including in muscle tissue. Biochemical signs of degenerative processes in muscle tissue have been identified as a result of simulation of paravertebral muscle ischemia. Key words. Muscle, disorders, degeneration, spine, mo­deling, hyperlipidemia, ligation, biochemistry.

Author Biographies

Volodymyr Radchenko, Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of Ukraine, Kharkiv

MD, Prof. in Traumatology and Orthopаedics

Frieda Leontyeva, Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of Ukraine, Kharkiv

PhD in Biol. Sci.

Vladyslav Tuliakov, Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of Ukraine, Kharkiv

Doctor in Pharmacy

Mykyta Skidanov, Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of Ukraine, Kharkiv

Intern doctor in Traumatology and Orthopaedics

Artem Skidanov, Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of Ukraine, Kharkiv

Doctor in Traumatology and Orthopаedics

Olga Nikolchenko, Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of Ukraine, Kharkiv

PhD in Biol. Sci.

References

  1. Fernández-Sada, E., Torres-Quintanilla, A., Silva-Platas, C., García, N., Willis, B. C., Rodríguez-Rodríguez, C., … & García-Rivas, G. (2017). Proinflammatory cytokines are soluble mediators linked with ventricular arrhythmias and contractile dysfunction in a rat model of metabolic syndrome. Oxidative Medicine and Cellular Longevity, 2017, 1-12. https://doi.org/10.1155/2017/7682569
  2. Radchenko, V. O., Skidanov, A. G., & Morozenko, D. V. (2017). Relative content of different tissues in the paravertebral muscles of the lumbar spine in conditions of degenerative diseases and in healthy depending on age. Orthopedics, Traumatology and Prosthetics, 1(606), 80–86. https://doi.org/10.15674/0030-59872017180-86. [in Ukrainian]
  3. Radchenko, V. O., Skidanov, A. G., & Morozenko, D. V. (2018). Dynamics of biochemical blood markers in patients after surgical treatment of degenerative diseases of the lumbar spine. Ukrainian Journal of Medicine, Biology and Sports, 7(16), 140–145. https://doi.org/10.26693/jmbs03.07.140. [in Ukrainian]
  4. Mehanna, E. T., El-sayed, N. M., Ibrahim, A. K., Ahmed, S. A., & Abo-Elmatty, D. M. (2018). Isolated compounds from Cuscuta pedicellata ameliorate oxidative stress and upregulate expression of some energy regulatory genes in high fat diet induced obesity in rats. Biomedicine & Pharmacotherapy, 108, 1253-1258. https://doi.org/10.1016/j.biopha.2018.09.126
  5. Crawford, R. J., Volken, T., Valentin, S., Melloh, M., & Elliott, J. M. (2016). Rate of lumbar paravertebral muscle fat infiltration versus spinal degeneration in asymptomatic populations: An age-aggregated cross-sectional simulation study. Scoliosis and Spinal Disorders, 11(1). https://doi.org/10.1186/s13013-016-0080-0
  6. Crossman, K., Mahon, M., Watson, P. J., Oldham, J. A., & Cooper, R. G. (2004). Chronic low back pain-associated Paraspinal muscle dysfunction is not the result of a constitutionally determined “Adverse” fiber-type composition. Spine, 29(6), 628-634. https://doi.org/10.1097/01.brs.0000115133.97216.ec
  7. Hides, J. A., Stokes, M. J., Saide, M., Jull, G. A., & Cooper, D. H. (1994). Evidence of lumbar Multifidus muscle wasting ipsilateral to symptoms in patients with acute/Subacute low back pain. Spine, 19(Supplement), 165-172. https://doi.org/10.1097/00007632-199401001-00009
  8. European Convention for the protection of vertebrate animals used for research and other scientific purposes. Strasbourg, 1986.
  9. Law of Ukraine № 3447-IV of February 21, 2006 “On Protection of Animals from Cruelty” (Article 26). https://zakon.rada.gov.ua/laws/show/3447-15#Text. [in Ukrainian]
  10. Kang, Y., Kim, S., & Kim, J. (2017). Effects of swimming exercise on high-fat diet-induced low bone mineral density and trabecular bone microstructure in rats. Journal of Exercise Nutrition & Biochemistry, 21(2), 48-55. https://doi.org/10.20463/jenb.2016.0063
  11. Leontyeva, F. S, &. Morozenko, D. V. (2016). Biochemical markers of connective tissue metabolism in osteochondrosis of the lumbar spine. Pivdennoukrainian Medical Science Journal, 13, 100–102. [in Russian]
  12. Tymoshenko, O. P., Voronina, L. M., & Kravchenko, V. M. (2003). Clinical biochemistry: textbook. Kharkiv: Golden Pages. [in Ukrainian]
  13. Vlizla, V. V. (2012). Laboratory research methods in biology, animal husbandry and veterinary medicine: handbook. Lviv: SPOLOM. [in Ukrainian]
  14. Kamyshnikov, V. S. (2003). Clinical and biochemical laboratory diagnostics. Reference book in 2 volumes. Minsk: Interservice. [in Russian]
  15. Lang, T. A., & Sesik, M. M. (2011). How to describe statistics in medicine. A guide for authors, editors and reviewers. Moscow: Practical Medicine. [in Russian]
  16. Fujimoto, S., Mochizuki, K., Shimada, M., Hori, T., Murayama, Y., Ohashi, N., & Goda, T. (2010). Insulin resistance induced by a high-fat diet is associated with the induction of genes related to leukocyte activation in rat peripheral leukocytes. Life Sciences, 87(23-26), 679-685. https://doi.org/10.1016/j.lfs.2010.10.001
  17. Cheng, H. S., Ton, S. H., Phang, S. C., Tan, J. B., & Abdul Kadir, K. (2017). Increased susceptibility of post-weaning rats on high-fat diet to metabolic syndrome. Journal of Advanced Research, 8(6), 743-752. https://doi.org/10.1016/j.jare.2017.10.002
  18. Mansouri, A., Gattolliat, C., & Asselah, T. (2018). Mitochondrial dysfunction and signaling in chronic liver diseases. Gastroenterology, 155(3), 629-647. https://doi.org/10.1053/j.gastro.2018.06.083
  19. Rissanen, A. (2004). Back muscles and intensive rehabilitation on patients with chronic low back pain. Effects on back muscle structure and function and patient disability [Doctoral dissertation]. http://urn.fi/URN:ISBN:951-39-2032-1.
  20. Belous, A. S., Biryukova, Yu. K., & Zatolokina, M. A. (2016). Trophic changes in the skeletal muscles of rats after pharmacotherapy with sildenafil and cerebrolysin in modeling lower limb ischemia. Bulletin of the Russian State Medical University, 4, 60–74. [in Russian]
  21. Lavrinenko, K. I., Mal, G. S., & Orlova, A. Yu. (2015). Experimental study of correction of chronic lower limb ischemia with vardenafil. Modern problems of science and education, 5. http://www.science-education.ru/ru/article/view?id=22060. [in Russian]
  22. Sukovatykh, B. S., Orlova, A. Yu., & Artyushkova, E. B. (2015). The effectiveness of the mononuclear fraction of autologous bone marrow in the treatment of experimental critical limb ischemia. Experimental surgery, 23(4), 365–371. [in Russian]

How to Cite

Radchenko, V. ., Leontyeva, F. ., Tuliakov, V. ., Skidanov, M. ., Skidanov, A. ., & Nikolchenko, O. . (2021). Modelling of degenerative changes in paravertebral muscles for studying of its influence on spine diseases. ORTHOPAEDICS TRAUMATOLOGY and PROSTHETICS, (1), 62–68. https://doi.org/10.15674/0030-59872021162-68

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Section

ORIGINAL ARTICLES