Vitamin D receptor gene polymorphism as a factor of early osteopenia formation in case of a combined course of osteoarthritis and obesity in young people

Ludmila Pasieshvili, Andrii Istomin, Konstantin Tereshkin, Olena Tereshkinа


Objective: to reveal the prognostic-diagnostic significance of polymorphism of vitamin D receptor (VDR) gene G63980A, reference SNP (rs) 1544410, and possible prognostication of the risk of disorders appearance in the structural-functional state of the bone tissue in a combined course of osteoarthritis (OA) and obesity in young people.

Methods: the study group included 96 patients at the age of (35.5 ± 0.9) years with OA and obesity, the control group were 96 practically healthy people of the same age and gender, the comparison group consisted of 18 patients with OA and the normal body mass.

Obesity was classified after calculation of the body mass (Ketle) index. The functional state of the locomotor system was assessed by the WOMAC scale. The mineral density of the bone tissue was measured with bone densitometer Explorer QDR W (Hologic). Gene polymorphism was investigated in the human genome by the method of polymerase chain reaction. Odds ratio (OR) of the disease development was calculated.

Results: severe radiological stages of joints were revealed 2.4 times more frequently in OA combined with obesity than in isolated OA. Osteoporosis was diagnosed in 59.4 % of patients from the study group versus 50 % in the comparison one. In the study group, patients with polymorphic variants of VDR gene, who had the B allele, predominated. The following risk factors for the development of OA were revealed: bone fractures in patients and their family members in the past history, genotype variation in VDR gene, age of the onset of disease under 30 and the radiological stage of OA.

Conclusions: clinical-instrumental manifestations of the disease worsen in young patients with OA against a background of obesity. The same patients form osteoporotic disorders as early as during five years of the combined course of the above nosologies. Prognostically unfavourable factors are as follows: the BB variant of VDR gene, fractures in patients and their family members’ past history, manifestation of the disease under 30 years of age and the radiological stage of OA.


osteoarthritis; obesity; vitamin D gene receptor; osteoporosis


The limited clinical utility of testosterone, estradiol, and sex

hormone binding globulin measurements in the prediction

of fracture risk and bone loss in older men / E. S. Orwoll,

J. Lapidus, P. Y. Wang [et al.] // Journal of Bone and Mineral

Research. — 2017. — Vol. 32 (3). — P. 633–640. —

DOI: 10.1002/jbmr.3021.

Geusens P. P. Osteoporosis and osteoarthritis shared mechanisms

and epidemiology / P. P. Geusens, J. P. van den Bergh //

Current Opinion in Rheumatology. — 2016. — Vol. 28 (2). —

P. 97–103. — DOI: 10.1097/BOR.0000000000000256.

Bone Density loss is associated with blood cell counts /

R. J. Valderrábano, L. Y. Lui, S. R. Cummings [et al.] // Journal

of Bone and Mineral Research. — 2017. — Vol. 32 (2). —

P. 212–220. — DOI: 10.1002/jbmr.3000.

Поворознюк В. В. Сучасний погляд на проблему остеопо-

розу у чоловіків в Україні / В. В. Поворознюк, Т. В. Орлик,

Є. О. Крєслов // Боль. Суставы. Позвоночник. — 2012. —

Т. 2, № 6. — С. 42–49.

Collaborative meta-analysis: associations of 150 candidate genes

with osteoporosis and osteoporotic fracture / J. B. Richards,

F. K. Kavvoura, F. Rivadeneira [et al.] // Annals of Internal

Medicine. — 2009. — Vol. 151 (8). — P. 528–537. —

DOI: 10.7326/0003-4819-151-8-200910200-00006.

Anti-inflammatory effect of 1,25-dihydroxyvitamin D3 is associated

with crosstalk between signal transducer and activator

of transcription 5 and the vitamin D receptor in human

monocytes / M. Yang, B. O. Yang, H. Gan [et al.] // Experimental

and Therapeutic Medicine. — 2015. — Vol. 9 (5). —

P. 1739–1744. — DOI: 10.3892/etm.2015.2321.

The association between common vitamin D receptor gene

variations and osteoporosis: a participantlevel metaanalysis /

A. G. Uitterlinden, S. H. Ralston, M. L. Brandi [et al.] // Annals

of Internal Medicine. — 2006. — Vol. 145 (4). — P. 255–264. —

DOI: 10.7326/0003-4819-145-4-200608150-00005.

Gao J. Influence of BsmI polymorphism in vitamin D receptor

gene on the risk of fracture in Caucasian populations:

a meta analysis / J. Gao, L. Wang, J. Zhu // International

Journal of Clinical and Experimental Medicine. — 2015. —

Vol. 8 (1). — P. 589–597.

Vitamin D Inhibits expression and activity of matrix metalloproteinase

in human lung fibroblasts (HFL-1) cells /

S. H. Kim, M. S. Baek, D. S. Yoon [et al.] // Tuberculosis

and Respiratory Diseases (Seoul). — 2014. — Vol. 77 (2). —

P. 73–80. — DOI: 10.4046/trd.2014.77.2.73.

American College of Rheumatology 2012 recommendations

for the use of non-pharmacologic and pharmacologic therapies

in osteoarthritis of the hand, hip, and knee / M. C. Hochberg,

R. D. Altman, K. T. April [et al.] // Arthritis Care & Research. —

— Vol. 64 (4). — P. 465–474. — DOI: 10.1002/acr.21596.

Клінічний протокол надання медичної допомоги хворим

із остеоартрозом (ОА). Додаток 6 до наказу Міністерства

о;хорони здоров’я України від 12.10.2006 № 676 // Український

ревматологічний журнал. — 2007. — № 27 (1). — С. 74–75.

Advances in NSAID development: evolution of diclofenac

products using pharmaceutical technology / R. Altman,

B. Bosch, K. Brune [et al.] // Drugs. — 2015. — Vol. 75 (8). —

P. 859–877. — DOI: 10.1007/s40265-015-0392-z.

Validity, reliability, and comparison of the WOMAC osteoarthritis

index and Lequesne algofunctional index in Turkish

patients with hip or knee osteoarthritis / S. Basaran, R. Guzel,

G. Seydaoglu, F. Guler-Uysal // Clin Rheumatol. — 2010. —

Vol. 29 (7). — P. 749–756.— DOI: 10.1007/s10067-010-1398-2.

Костная денситометрия в клинической практике / В. А. Рад-

ченко, С. Б. Костерин, Н. В. Дедух, Е. А. Побел // Ортопе-

дия, травматология и протезирование. — 2015. — № 2. —

C. 100–108. — DOI: 10.15674/0030-598720152100-107.

Кобзарь А. И. Прикладная математическая статистика.

Для инженеров и научных работников / А. И. Кобзарь. —

-е изд., испр. — М. : Физматлит, 2012. — 816 с.

Картель Н. А. Генетика. Энциклопедический словарь:

словарь / Н. А. Картель, Е. Н. Макеева, А. М. Мезенко. —

Минск : Белорусская наука, 2011 — 992 с.

Гублер Е. В. Информатика в патологии, клинической медицине

и педиатрии / Е. В. Гублер. — Л. : Медицина, 1990. — 176 с.

Гублер Е. В. Вычислительные методы анализа и распоз-

навания патологических процессов / Е. В. Гублер. — Л. :

Медицина, 1978. — 296 c.

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