A modification of superficial properties of materials by means of application of multiple coatings for their use in orthopaedics

Oleg Vyrva, Anna Zykova, Vladimir Safonov, Svitlana Malyshkina, Volodimir Lukyanchenko, Yadvihа Valkovich, Renata Rogowska, Stanislav Yakovin


The article analyses studies of surface parameters of different multiple coatings, such as: TiN, CrN, (Ti,Cr)N, TiN/TiC10N90, TiN/TiC20N80, which are applied by the arc method (Arc-PVD) on stainless steel samples (1H18N9), and the same coatings with an additional oxide layer Al2O3, which are deposited by the method of reactive magnetron sputtering (RMS method). A comparative analysis of the thickness, roughness and structural characteristics of surfaces was carried on. Other parameters, such as surface wettability (water receptivity / water repellency), surface free energy (SFE) and fractional polarity, were determined by methods of Wu, Owens-Wendt-Rabel-Kaelble, Van Oss and Fowkes. In order to make a further conclusion about an effect of the above factors on biological objects, in vitro experiments were carried out for studying relationships between cell structures and the material in fibroblast cultures. The results show some correlation between the surface properties of the materials and adhesion and proliferation of the cell cultures. Better biological parameters of the response (the total number of cells, proliferation, morphology of cells) were received in case of coatings with higher values of the polar components of SFE and fractional polarity.


coatings for biomaterials; surface parameters; cell responses


Mandl S. Improvement the biocompatibility of medical implants with plasma immersion ion implantation [Text] / S. Mandl, B. Rauchenbach // J. Surface&Coating Technology. — 2002. — Vol. 156. — P. 583–589.

S180 cell growth on low energy plasma treated TiO2 thin films [Text] / M. Dhayal, S.I. Cho, J.Y. Moon et al. // J. Applied Surface Sci.— 2008. — Vol. 254. — P. 3331–3338


Basic reactions of osteoblasts on structured material surface [Text] / U. Meyer, A. Bushter, H.P. Wiesmann et al. // J. Eur. Cells Mater. — 2005. — Vol. 9. — P. 39–49.

Anselme K. Osteoblast adhesion on biomaterials [Text] / K. Anselme // Biomaterials. — 2000. — Vol. 21. — P. 667–681.

Attachment and proliferation of osteoblasts and fibroblasts on biomaterial for orthopedic use [Text] / A. Hunter, C.W. Archer, P.S. Walker, G.W. Blunn // Biomaterials. — 1995. — Vol. 16. — P. 287–295.

Responce of MG63osteoblast-like cells to titanium and titanium alloy is depend on surface roughness and composition [Text] / J.Links, B.D. Boyan, C.R. Blanchard et al. // Biomaterials. — 1998. — Vol. 19. — P. 2219–2232.

Response of rat bone marrow cells to commercially pure titanium submitted to different surface treatments [Text] / S.P. Xavier, P.S.P. Carvalho, M.M. Beloti, A.L. Rosa // J. Dent. — 2003. — Vol. 31. — P. 173–180.

Interactions of fibroblast and polymer surfaces, relation between surface free energy and fibroblast spreading [Text] / P. Van der Valk, A.W.J. van Pelt, H.J. Busscher, H.P.J. de Long // Biomed. Mater. Res. — 1983. — Vol. 17. — P. 807–817.

Zykova A.V. The corrosion properties of implanted materials with protective coatings in isotonic physiological solution [Text] / A.V. Zykova, V.V. Luk’yanchenko, V.I. Safonov // J. Surface&Coating Technology. — 2005. — Vol. 200. — P. 90–93.

The influence of surface parameters of coatings deposited by various vacuum-plasma methods on the cell/material interaction in vitro tests [Text] / A. Zykova, V. Safonov, V. Luk’yanchenko et al. // Problems of Atomic Science and Technology Series: Plasma Physics. — 2007. — Vol. 13. — P. 200–202.

The influence of deposition technological process parameters on surface properties of multilayer coatings [Text] / A. Zykova, V. Safonov, J. Walkowicz et al. // Problems of Atomic Science and Technology Series: Plasma Physics. — 2009. — Vol. 15. — P. 159–161.

Multifunctional ToF-SIMS combinatorial mapping of gradient energy substrates [Text] / S.V. Roberson, A.J. Fahey, A. Sehgal, A. Karim // J. Applied Surface Sci. — 2002. — Vol. 200. — P. 150–164.

Combinatorial screen of effect of surface energy on fibronectin mediated osteoblast adhesion, spreading and proliferation [Text] / S.B. Kennedy, R.N. Washburn, C.G. Simon, E.J. Amis // Biomaterials. — 2006. — Vol. 27. — P. 3817–3824.

Evolution of metallic and polymeric surface energy and surface roughness characteristics for directed cell adhesion [Text] / N.J. Hallab, K.J. Bundy, K.J. O’Connor et al. // Tissue Engineering. — 2001. — Vol. 7. — P. 55–71.

Surface parameters modification by multilayer coating deposition for biomedical applications [Text] / A. Zykova, V. Safonov, O. Vyrva et al. // Journal of Physics Conference Series. — 2008. — Vol. 113. — P. 012–029.

Copyright (c) 2014 Oleg Vyrva, Anna Zykova, Vladimir Safonov, Svitlana Malyshkina, Volodimir Lukyanchenko, Yadvihа Valkovich, Renata Rogowska, Stanislav Yakovin

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.