DOI: https://doi.org/10.15674/0030-59872018366-73
Open Access Open Access  Restricted Access Subscription Access

Morphological changes of the distal femur growth plate of rabbits under bilateral temporal block using non-locking plates and screws

Sergey Khmyzov, Victor Rokutov, Dmytro Iershov, Nataliya Ashukina, Zinaida Danyshchuk, Valentyna Maltseva

Abstract


For the treatment of the moderate leg length discrepancy (2–6 cm) in children surgeons use temporal growth plate (GP or epiphyseal cartilage) block with plates and screws.

Objective: to study morphological changes in distal femur growth plate of rabbits under bilateral temporal block with non-locking plates and screws.

Methods: we blocked distal GP of the right femur of 9 rabbits (8 weeks old). On 3rd, 5th and 7th weeks histological study of the distal femoral growth plate of both femurs with morphometry was made.

Results: in 3rd weeks after surgery the height of the GP on the operated side was decreased in lateral and medial parts in 2.06 and 1.98 times (p < 0.001), in central — conversely — increased in 1.18 times (p < 0.001) compare to contralateral limb. In 5th weeks in the whole GP structural changes were noted. Height of the GP was decreased comparing to contralateral limb in the lateral side in 1.3 times (p < 0.001), in the medial part — in 1.14 (p < 0.01) times. On 7th week destructive changes in the GP progressed, its height was decreased comparing to contralateral limb in the lateral part in 3.29 times, in the medial — in 3.5 times (p < 0.001).

Conclusions: on 3rd, 5th, 7th weeks after bilateral distal femur GP block in rabbits we have found all typical zones. Destructive changes (histoarchitecture disorder, cells density etc.) progressed during the time of experiment. The height of the operated distal femoral GP and area of the primary osteogenesis was decreased comparing to the contralateral kimb with longer follow-up period. It indicates an inhibition of the longitudinal bone growth.


Keywords


epiphyseal cartilage; growth plate; temporary bilateral block; experiment; histology

References


European convention for the protection of vertebral animals used for experimental and other scientific purposes. Strasbourg, March 18, 1986: Official translation [Electronic resource] / Verkhovna Rada of Ukraine. — Official website. — (International Council of Europe document). — Access to the document: http://zakon.rada.gov.ua/cgi-bin/laws/main. cginreg=994_137. (in Ukrainian)

Iershov, D. V. (2016). Experimental and clinical justification of unilateral growth plate blocking for pediatric frontal knee deformities treatment: dissertation for the scientific degree of the candidate of medical sciences, «Traumatology and Orthopedics». Kharkiv. (in Ukrainian)

About animal’s protection from cruel treatment: The Law of Ukraine № 3447-IV of 21.02. 2006. The Verkhovna Rada of Ukraine. Official website. Retrieved from: http://zakon.rada.gov.ua/cgi-bin/laws/ main.cgi?nreg=3447-15. (in Ukrainian)

Sarkisov, D., Perov, J. (1996). Microscopic technic. Moscow: Medicine. (in Russian)

Khmyzov, S., Rokutov, V., & Iershov, D. (2017). Development of the distal femur metaepiphysis during temporary bilateral blocking of the growth plate using different types of plates: an experimental study. Orthopaedics, traumatology and prosthetics, 3, 48–53. doi: http://dx.doi.org/10.15674/0030-59872017348-53. (in Ukrainian)

A. Ahmed, Y., Abdelrahim, E. A., & Khalil, F. (2015). Histological sequences of long bone development in the new zealand white rabbits. Journal of Biological Sciences, 15(4), 177–186. doi:10.3923/jbs.2015.177.186

Chung, R., & Xian, C. J. (2014). Recent research on the growth plate: Mechanisms for growth plate injury repair and potential cell-based therapies for regeneration. Journal of Molecular Endocrinology, 53(1), T45–T61. doi:10.1530/jme-14-0062

Gottliebsen, M., Møller-Madsen, B., Stødkilde-Jørgensen, H., & Rahbek, O. (2013). Controlled longitudinal bone growth by temporary tension band plating. The Bone & Joint Journal, 95-B(6), 855–860. doi:10.1302/0301-620x.95b6.29327

Eastwood, D. M., & Sanghrajka, A. P. (2011) Guided growth: recent advances in a deep-rooted concept. Journal Bone and Joint Surgery Br. 93(1). 12–18. doi: 10.1302/0301-620X.93B1.25181

Eastwood, D. M., & Sanghrajka, A. P. (2011). Guided growth. The Journal of Bone and Joint Surgery. British volume, 93-B(1), 12–18. doi:10.1302/0301-620x.93b1.25181

Ghanem, I., Karam, J. A., & Widmann, R. F. (2011). Surgical epiphysiodesis indications and techniques: update. Current Opinion in Pediatrics, 23(1), 53–59. doi:10.1097/mop.0b013e32834231b3

Gofton, J. P. (1985) Persistent low back pain and leg length disparity. The Journal of Rheumatology, 12(4), 747–750.

Gottliebsen, M. (2013). Guided growth of long bones using the tension band plating technique: Experimental and clinical studies : PhD thesis. Aarhus: Health, Aarhus Universitet.

Gottliebsen, M., Shiguetomi-Medina, J. M., Rahbek, O., & Møller-Madsen, B. (2016). Guided growth: mechanism and reversibility of modulation. Journal of Children's Orthopaedics, 10(6), 471–477. doi:10.1007/s11832-016-0778-9

Iannotti, J. P. (1990) Growth plate physiology and pathology. Orthopedic Clinics of North America, 21, 1–17.

Karbowski, A., Camps, L., & Matthia, H. H. (1989). Histopathological features of unilateral stapling in animal experiments. Archives of Orthopaedic and Trauma Surgery, 108(6), 353–358. doi:10.1007/bf00932445

Cheon, J., Kim, I., Choi, I. H., Kim, C. J., Cho, T., Kim, W. S., … Yeon, K. M. (2005). Magnetic resonance imaging of remaining physis in partial physeal resection with graft interposition in a rabbit model. Investigative Radiology, 40(4), 235–242. doi:10.1097/01.rli.0000157316.20075.8e

Pendleton, A. M., Stevens, P. M., & Hung, M. (2013). Guided growth for the treatment of moderate leg-length discrepancy. Orthopedics, 36(5), e575-e580. doi:10.3928/01477447-20130426-18

Kömür, B. (2013). Permanent and temporary epiphysiodesis: an experimental study in a rabbit model. Acta Orthopaedica et Traumatologica Turcica, 47(1), 48–54. doi:10.3944/aott.2013.2949

Gaumétou, E., Mallet, C., Souchet, P., Mazda, K., & Ilharreborde, B. (2016). Poor efficiency of eight-plates in the treatment of lower limb discrepancy. Journal of Pediatric Orthopaedics, 36(7), 715–719. doi:10.1097/bpo.0000000000000518

Ross, T. K., & Zionts, L. E. (1997). Comparison of different methods used to inhibit physeal growth in a rabbit model. Clinical Orthopaedics and Related Research, 340, 236–243. doi:10.1097/00003086-199707000-00031

Rossvoll, I., Junk, S., & Terjesen, T. (1992). The effect on low back pain of shortening osteotomy for leg length inequality. International Orthopaedics, 16(4). doi:10.1007/bf00189625

Stevens, P. M. (2007). Guided growth for angular correction. Journal of Pediatric Orthopaedics, 27(3), 253–259. doi:10.1097/bpo.0b013e31803433a1

Stevens, P. M. (2016). The role of guided growth as it relates to limb lengthening. Journal of Children's Orthopaedics, 10(6), 479–486. doi:10.1007/s11832-016-0779-8

Stewart, D., Cheema, A., & Szalay, E. A. (2013). Dual 8-plate technique is not as effective as ablation for epiphysiodesis about the knee. Journal of Pediatric Orthopaedics, 33(8), 843–846. doi:10.1097/bpo.0b013e3182a11d23

Tomaszewski, R., Gap, A., & Wiktor, L. (2017). Histological evaluation in autologous growth plate chondrocyte grafting in rabbits. Journal of Cytology & Histology, 08(04). doi:10.4172/2157-7099.1000472




Copyright (c) 2018 Sergey Khmyzov, Victor Rokutov, Dmytro Iershov, Nataliya Ashukina, Zinaida Danyshchuk, Valentyna Maltseva

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.