The dynamics of blood indexes in rats after ceramic biomaterial implantation in defects of the femur methaphis and diaphysis


  • Vasyl Shimon
  • Yurii Meklesh



bone, rats, methaphis, diaphysis, regeneration, bioceramic material, implantation


Objective: on the base of blood markers dynamics we determined the influence of ceramic material on the rats’ organism and bone regeneration after implantation in the femur methaphis and diaphysis.

Methods: study was made on rats male, age of 4.5 months in 4 groups with 9 rats in each group. The 1st group — the methaphis femur defect was empty, in the 2nd group — the defect was filled with ceramic material. The 3rd and 4th g roups – diaphysis d efects were made correspondently. For implantation we used ceramic material consisted of 57.77 % of hydroxyapatite and 47.23 % of three­calciumphosphate beta. In 7, 14, 28, 56 days clinical and biochemistry analyses were made.

Results: biochemistry indexes of the liver functional state, glucose, urea did not change after implantation of ceramic material. In 7 days after making of defect in the femur we have found moderate leukocytosis. After ceramic material implantation in rats indexes of hematology analysis did not change. We have found decreasing of creatinine level in 7 days in all groups: in the 1st — 28.2 % , in the 2nd — 29.2 %, in the 3rd — 21.6 %, in the 4th — 21.9 %. Glycoproteins, chondroitin sulfates, alkaline phosphatase activity in blood plasma have shown the revitalization of regeneration process on early stages and these indexes were decreased in late follow-up, it was more pronounced after ceramic material implantation. In 56 days the blood indexes did not differ from the data obtained from intact rats.

Conclusions: it was found that after implantation of ceramic material there was not toxic effect on rats’ organism. Indexes of glycoproteins, chondroitin sulfates, alka­line phosphatase activity found in blood testified of more pronounced regeneration in the place of bone defect with ceramic material implantation.

Author Biographies

Vasyl Shimon

Uzhhorod National University. Ukraine 

MD, Prof. in Traumatology and Orthopaedics

Yurii Meklesh

Uzhhorod National University. Ukraine


Frasca, S., Norol, F., Le Visage, C., Collombet, J., Letourneur, D., Holy, X., & Sari Ali, E. (2017). Calcium-phosphate ceramics and polysaccharide-based hydrogel scaffolds combined with mesenchymal stem cell differently support bone repair in rats. Journal of Materials Science: Materials in Medicine, 28(2). doi:10.1007/s10856-016-5839-6

Gao, C., Deng, Y., Feng, P., Mao, Z., Li, P., Yang, B., … Peng, S. (2014). Current progress in bioactive ceramic scaffolds for bone repair and regeneration. International Journal of Molecular Sciences, 15(3), 4714-4732. doi:10.3390/ijms15034714

Maté-Sánchez de Val, J. E., Mazón, P., Calvo-Guirado, J. L., Ruiz, R. A., Ramírez Fernández, M. P., Negri, B., … De Aza, P. N. (2013). Comparison of three hydroxyapatite/β-tricalcium phosphate/collagen ceramic scaffolds: An in vivo study. Journal of Biomedical Materials Research Part A, 102(4), 1037-1046. doi:10.1002/jbm.a.34785

Fabris, A. L., Faverani, L. P., Gomes-Ferreira, P. H., Polo, T. O., Santiago-júnior, J. F., & Okamoto, R. (2018). Bone repair access of BoneCeramic™ in 5-mm defects: study on rat calvaria. Journal of Applied Oral Science, 26(0). doi:10.1590/1678-7757-2016-0531

Yu, T., Pan, H., Hu, Y., Tao, H., Wang, K., & Zhang, C. (2017). Autologous platelet-rich plasma induces bone formation of tissue-engineered bone with bone marrow mesenchymal stem cells on beta-tricalcium phosphate ceramics. Journal of Orthopaedic Surgery and Research, 12(1). doi:10.1186/s13018-017-0665-1

Nakamura, S., Ito, H., Nakamura, K., Kuriyama, S., Furu, M., & Matsuda, S. (2017). Long-term durability of ceramic tri-condylar knee implants: a minimum 15-year follow-up. The Journal of Arthroplasty, 32(6), 1874–1879. doi:10.1016/j.arth.2017.01.016

Nguyen, T., Bae, T., Yang, D., Park, M., & Yoon, S. (2017). Effects of titanium mesh surfaces-coated with hydroxyapatite/β-tricalcium phosphate nanotubes on acetabular bone defects in rabbits. International Journal of Molecular Sciences, 18(7), 1462. doi:10.3390/ijms18071462

Shokrollahi, H., Salimi, F., & Doostmohammadi, A. (2017). The fabrication and characterization of barium titanate/akermanite nano-bio-ceramic with a suitable piezoelectric coefficient for bone defect recovery. Journal of the Mechanical Behavior of Biomedical Materials, 74, 365-370. doi:10.1016/j.jmbbm.2017.06.024

Vlizla, V. V. (2012). Laboratory methods of research in biology, livestock and veterinary medicine: a guide. Lviv: SPOLOM. (in Ukrainian)

Goryachkovsky, A. M. (2005). Clinical biochemistry in laboratory diagnostics.Odessa: Ecology. (in Russian)

Morozenko, D. V., & Leont'eva, F. S. (2016). Methods of dosage of markers in the metabolism of spinal tissue in the patient's clinical and experimental medical. Molody Vcheny: science magazine, 2(29), 168–172. (in Ukranian)

Glantz, S. (1998). Medical and Biological Statistics. Мoscow: Practice. (in Russian)

Giachelli, C. M., & Steitz, S. (2000). Osteopontin: a versatile regulator of inflammation and biomineralization. Matrix Biology, 19(7), 615-622. doi:10.1016/s0945-053x(00)00108-6

Sase, S. P., Ganu, J. V., & Nagane N. (2012). Osteopontin: a novel protein molecule. Indian Medical Gazette, 62–66.