The dynamics of blood indexes in rats after ceramic biomaterial implantation in defects of the femur methaphis and diaphysis

Authors

  • Vasyl Shimon Ужгородський національний університет. Україна, Ukraine
  • Yurii Meklesh Uzhhorod National University. Ukraine , Ukraine

DOI:

https://doi.org/10.15674/0030-598720182108-115

Keywords:

bone, rats, methaphis, diaphysis, regeneration, bioceramic material, implantation

Abstract

Objective: on the base of blood markers dynamics we determined the influence of ceramic material on the rats’ organism and bone regeneration after implantation in the femur methaphis and diaphysis.

Methods: study was made on rats male, age of 4.5 months in 4 groups with 9 rats in each group. The 1st group — the methaphis femur defect was empty, in the 2nd group — the defect was filled with ceramic material. The 3rd and 4th g roups – diaphysis d efects were made correspondently. For implantation we used ceramic material consisted of 57.77 % of hydroxyapatite and 47.23 % of three­calciumphosphate beta. In 7, 14, 28, 56 days clinical and biochemistry analyses were made.

Results: biochemistry indexes of the liver functional state, glucose, urea did not change after implantation of ceramic material. In 7 days after making of defect in the femur we have found moderate leukocytosis. After ceramic material implantation in rats indexes of hematology analysis did not change. We have found decreasing of creatinine level in 7 days in all groups: in the 1st — 28.2 % , in the 2nd — 29.2 %, in the 3rd — 21.6 %, in the 4th — 21.9 %. Glycoproteins, chondroitin sulfates, alkaline phosphatase activity in blood plasma have shown the revitalization of regeneration process on early stages and these indexes were decreased in late follow-up, it was more pronounced after ceramic material implantation. In 56 days the blood indexes did not differ from the data obtained from intact rats.

Conclusions: it was found that after implantation of ceramic material there was not toxic effect on rats’ organism. Indexes of glycoproteins, chondroitin sulfates, alka­line phosphatase activity found in blood testified of more pronounced regeneration in the place of bone defect with ceramic material implantation.

Author Biographies

Vasyl Shimon, Ужгородський національний університет. Україна

MD, Prof. in Traumatology and Orthopaedics 

 

Yurii Meklesh, Uzhhorod National University. Ukraine 

 

 

References

  1. Frasca, S., Norol, F., Le Visage, C., Collombet, J., Letourneur, D., Holy, X., & Sari Ali, E. (2017). Calcium-phosphate ceramics and polysaccharide-based hydrogel scaffolds combined with mesenchymal stem cell differently support bone repair in rats. Journal of Materials Science: Materials in Medicine, 28(2). doi:https://doi.org/10.1007/s10856-016-5839-6
  2. Gao, C., Deng, Y., Feng, P., Mao, Z., Li, P., Yang, B., … Peng, S. (2014). Current progress in bioactive ceramic scaffolds for bone repair and regeneration. International Journal of Molecular Sciences, 15(3), 4714-4732. doi:https://doi.org/10.3390/ijms15034714
  3. Maté-Sánchez de Val, J. E., Mazón, P., Calvo-Guirado, J. L., Ruiz, R. A., Ramírez Fernández, M. P., Negri, B., … De Aza, P. N. (2013). Comparison of three hydroxyapatite/β-tricalcium phosphate/collagen ceramic scaffolds: An in vivo study. Journal of Biomedical Materials Research Part A, 102(4), 1037-1046. doi:https://doi.org/10.1002/jbm.a.34785
  4. Fabris, A. L., Faverani, L. P., Gomes-Ferreira, P. H., Polo, T. O., Santiago-júnior, J. F., & Okamoto, R. (2018). Bone repair access of BoneCeramic™ in 5-mm defects: study on rat calvaria. Journal of Applied Oral Science, 26(0). doi:https://doi.org/10.1590/1678-7757-2016-0531
  5. Yu, T., Pan, H., Hu, Y., Tao, H., Wang, K., & Zhang, C. (2017). Autologous platelet-rich plasma induces bone formation of tissue-engineered bone with bone marrow mesenchymal stem cells on beta-tricalcium phosphate ceramics. Journal of Orthopaedic Surgery and Research, 12(1). doi:https://doi.org/10.1186/s13018-017-0665-1
  6. Nakamura, S., Ito, H., Nakamura, K., Kuriyama, S., Furu, M., & Matsuda, S. (2017). Long-term durability of ceramic tri-condylar knee implants: a minimum 15-year follow-up. The Journal of Arthroplasty, 32(6), 1874–1879. doi:https://doi.org/10.1016/j.arth.2017.01.016
  7. Nguyen, T., Bae, T., Yang, D., Park, M., & Yoon, S. (2017). Effects of titanium mesh surfaces-coated with hydroxyapatite/β-tricalcium phosphate nanotubes on acetabular bone defects in rabbits. International Journal of Molecular Sciences, 18(7), 1462. doi:https://doi.org/10.3390/ijms18071462
  8. Shokrollahi, H., Salimi, F., & Doostmohammadi, A. (2017). The fabrication and characterization of barium titanate/akermanite nano-bio-ceramic with a suitable piezoelectric coefficient for bone defect recovery. Journal of the Mechanical Behavior of Biomedical Materials, 74, 365-370. doi:https://doi.org/https://doi.org/10.1016/j.jmbbm.2017.06.024
  9. Vlizla, V. V. (2012). Laboratory methods of research in biology, livestock and veterinary medicine: a guide. Lviv: SPOLOM. (in Ukrainian)
  10. Goryachkovsky, A. M. (2005). Clinical biochemistry in laboratory diagnostics.Odessa: Ecology. (in Russian)
  11. Morozenko, D. V., & Leont'eva, F. S. (2016). Methods of dosage of markers in the metabolism of spinal tissue in the patient's clinical and experimental medical. Molody Vcheny: science magazine, 2(29), 168–172. (in Ukranian)
  12. Glantz, S. (1998). Medical and Biological Statistics. Мoscow: Practice. (in Russian)
  13. Giachelli, C. M., & Steitz, S. (2000). Osteopontin: a versatile regulator of inflammation and biomineralization. Matrix Biology, 19(7), 615-622. doi:https://doi.org/10.1016/s0945-053x(00)00108-6
  14. Sase, S. P., Ganu, J. V., & Nagane N. (2012). Osteopontin: a novel protein molecule. Indian Medical Gazette, 62–66.

Downloads

How to Cite

Shimon, V., & Meklesh, Y. (2023). The dynamics of blood indexes in rats after ceramic biomaterial implantation in defects of the femur methaphis and diaphysis. ORTHOPAEDICS TRAUMATOLOGY and PROSTHETICS, (2), 108–115. https://doi.org/10.15674/0030-598720182108-115

Issue

No. 2 (2018)

Section

ORIGINAL ARTICLES

License

Copyright (c) 2018 Vasyl Shimon, Yurii Meklesh

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

The authors retain the right of authorship of their manuscript and pass the journal the right of the first publication of this article, which automatically become available from the date of publication under the terms of Creative Commons Attribution License, which allows others to freely distribute the published manuscript with mandatory linking to authors of the original research and the first publication of this one in this journal.

Authors have the right to enter into a separate supplemental agreement on the additional non-exclusive distribution of manuscript in the form in which it was published by the journal (i.e. to put work in electronic storage of an institution or publish as a part of the book) while maintaining the reference to the first publication of the manuscript in this journal.

The editorial policy of the journal allows authors and encourages manuscript accommodation online (i.e. in storage of an institution or on the personal websites) as before submission of the manuscript to the editorial office, and during its editorial processing because it contributes to productive scientific discussion and positively affects the efficiency and dynamics of the published manuscript citation (see The Effect of Open Access).