The indexes of osteogenic stem cells activity in patients with rheumatoid arthritis and frontal knee deformities

Authors

  • Lesya Panchenko SI «Institute of Traumatology and Orthopaedics of the National Academy of Medical Sciences of Ukraine», Kyiv , Ukraine
  • Yevgen Avtomeenko SI «Institute of Traumatology and Orthopaedics of the National Academy of Medical Sciences of Ukraine», Kyiv , Ukraine
  • Andrey Babko SI «Institute of Traumatology and Orthopaedics of the National Academy of Medical Sciences of Ukraine», Kyiv , Ukraine
  • Sergey Gerasimenko SI «Institute of Traumatology and Orthopaedics of the National Academy of Medical Sciences of Ukraine», Kyiv , Ukraine
  • Andrey Gerasimenko SI «Institute of Traumatology and Orthopaedics of the National Academy of Medical Sciences of Ukraine», Kyiv, Ukraine
  • Vera Polishko SI «Institute of Traumatology and Orthopaedics of the National Academy of Medical Sciences of Ukraine», Kyiv , Ukraine

DOI:

https://doi.org/10.15674/0030-59872018141-46

Keywords:

osteogenic activity, frontal deformity, knee joint

Abstract

Objective: to study the indexes of stem cells activity of bone marrow which form knee joint (lateral and medial parts of distal femur and proximal tibia part) which were cut at arthroplasty surgery in patients with rheumatoid arthritis and frontal knee deformity.

Methods: spongiest bone was taken at surgery (knee arthroplasty) from medial and lateral sides of distal femur and proximal tibia. Due to cloning method of fibroblast colony forming units we studied in 37 patients with rheumatoid arthritis of knee joint. We studied 168 samples of bone marrow, 204 cultures of stem cells were growth.

Results: we observed significant deceleration of reparative process in bone tissue at rheumatoid arthritis. Significant difference of fibroblast colony forming units in 1 sm 3 volume in different parts of knee joint was observed. In the medial femur condylar this index was (0,2307 ± 0,16) · 104, it exceeds values obtained in other areas in 11–20 times. It was shown that stem cells of the lateral tibia condylar had worse conditions for regenerative potential in cases of valgus deformity compare with varus. There were 4 times less amount of fibroblast colony forming units and the efficacy of its cloning was 2.6 times lower.

Conclusion: significant differences were revealed in the indexes of osteogenic activity of stem cells which form knee joint (lateral and medial parts of distal femur and proximal tibia part).

Author Biographies

Lesya Panchenko, SI «Institute of Traumatology and Orthopaedics of the National Academy of Medical Sciences of Ukraine», Kyiv 

PhD 

 

Yevgen Avtomeenko, SI «Institute of Traumatology and Orthopaedics of the National Academy of Medical Sciences of Ukraine», Kyiv 

 

 

Andrey Babko, SI «Institute of Traumatology and Orthopaedics of the National Academy of Medical Sciences of Ukraine», Kyiv 

MD 

 

Sergey Gerasimenko, SI «Institute of Traumatology and Orthopaedics of the National Academy of Medical Sciences of Ukraine», Kyiv 

MD, Prof. 

 

Andrey Gerasimenko, SI «Institute of Traumatology and Orthopaedics of the National Academy of Medical Sciences of Ukraine», Kyiv

PhD 

Vera Polishko, SI «Institute of Traumatology and Orthopaedics of the National Academy of Medical Sciences of Ukraine», Kyiv 

 

 

References

  1. Ganzha, I. M., Kovalenko, V. M., Lysenko, G. I., & Svintsitsky, A. S. (1996). Rheumatology. Kyiv: Health.
  2. Gerasimenko, S. I. (2000). Orthopedic treatment of rheumatoid arthritis with lesions of the lower limbs. Kyiv: Press of Ukraine.
  3. Maniwa, K., Ishibashi, Y., Tsuda, E., Yamamoto, Y., Inoue, R., & Otsuka, H. (2013). Accuracy of image-free computer navigated total knee arthroplasty is not compromised in severely deformed varus knees. The Journal of Arthroplasty, 28(5), 802-806. doi: https://doi.org/10.1016/j.arth.2012.09.014
  4. Manrique Arija, S., López Lasanta, M., Jiménez Núñez, F. G., Ureña, I., Espiño-Lorenzo, P., Romero Barco, C. M., … Fernández Nebro, A. (2011). Annual trends in knee and hip arthroplasty in rheumatoid arthritis 1998–2007. Reumatología Clínica (English Edition), 7(6), 380-384. doi: https://doi.org/10.1016/j.reuma.2011.05.012
  5. Gerasimenko, S. I. (1997). Complex orthopedic treatment of patients with rheumatoid arthritis with lesions of the lower extremities (Doctoral dissertation). Kiev.
  6. Chernyak, V. P. (2008). Endoprosthetics of the knee joint in patients with rheumatoid arthritis (PhD thesis). Kiev.
  7. Friedenstein, A. Ya., & Lalykina, K. S. (1973). Bone tissue induction and osteogenic precursor cells. Moscow: Medicine.
  8. Astakhov, V. S, Rybachuk, O. I., Panchenko, L. M., & Torchinsky V. P. (2000). Microcirculation of bone marrow precursor cells in patients with joint damage. Immunology and Allergology, 1, 4–7.

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How to Cite

Panchenko, L., Avtomeenko, Y., Babko, A., Gerasimenko, S., Gerasimenko, A., & Polishko, V. (2023). The indexes of osteogenic stem cells activity in patients with rheumatoid arthritis and frontal knee deformities. ORTHOPAEDICS TRAUMATOLOGY and PROSTHETICS, (1), 41–46. https://doi.org/10.15674/0030-59872018141-46

Issue

No. 1 (2018)

Section

ORIGINAL ARTICLES

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Copyright (c) 2018 Lesya Panchenko, Yevgen Avtomeenko, Andrey Babko, Sergey Gerasimenko, Andrey Gerasimenko, Vera Polishko

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