Regeneration of full-length layers cartilage in experimental under the impaction platelet rich plasma


  • Sergiy Vernygorodskyi
  • Volodymyr Fishchenko
  • Maksym Rybinskyi



platelet rich plasma, growth factors, local cartilage defects, experiment


It is known that even small damage to the cartilage, regard­less of genesis, is not spontaneously repaired. This provokes in the future the spread of degenerative joint damage and the oc­currence of irreversible secondary arthrosis changes.

Objective: to analyze the effect of autologous platelet rich plasma on the re­generation process in simulated cartilage defects in the intercon­dylar region of the femur in rabbits, to evaluate the microscopic pattern at different time intervals, to identify possible disadvan­tages and complications of the technique.

Methods: the experi­ment was conducted on 24 adult rabbits. Defects were created in the intercondylar area of the distal femur of both extremities: with involvement of the subchondral plate before the appearance of blood dew — 12 defects in the main group and 10 in the con­trol group, without its involvement — 12 and 10, respectively. On the 7th d ay a fter t he o peration, p latelet r ich p lasma w as injected into the joints of the control group rabbits. In 2 ani­mals (intact), the knee joints were not injured. After 2, 4 and 8 weeks, a macroscopic and histological analysis of the formed regenerate was carried out.

Results: 8 weeks after the operation, the average thickness of the regenerate in animals with damage to the subchondral plate was (0.34 ± 0.06) mm in the main group and (0.58 ± 0.009) mm in the control group. Without damage to the subchondral plate, the regeneration of the cartilaginous tissue is limited and after 8 weeks is practically not observed even when using platelet rich plasma. Regenerate in the main group was a fibrous-cartilaginous structure with signs of dif­ferentiation of chondroblasts, in control — fibrous, with a large number of vessels.

Conclusion: the structure of the regenerate in the defects of the articular cartilage with the involve­ment of the subchondral plate proved to be qualitatively better in the subgroup of animals that received platelet rich plasma in the postoperative period. 

Author Biographies

Sergiy Vernygorodskyi

National Pirogov Memorial Medical University, Vinnytsya. Ukraine 

MD, Prof.

Volodymyr Fishchenko

National Pirogov Memorial Medical University, Vinnytsya. Ukraine 

MD, Prof.

Maksym Rybinskyi

National Pirogov Memorial Medical University, Vinnytsya. Ukraine


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