Pathomorphological changes in traumatic defects of the articular surface of the tibial condyles after implantation of autogenic chondrocytes, which were cultured ex vivo in experiment


  • Valeriy Hryhorovskyi
  • Sergey Strafun
  • Oleg Kostogryz
  • Dmitro Zubov



traumatic defect, articular cartilage, cultivated autogenic chondrocytes, pathomorphological changes, experiment


The purpose of this experimental study was to obtain results of the reparative process and morphometric parameters of articular surface tissues of the knee joint after their mechanical injury and a local implantation of an autochondrocyte (autoCC) culture on a carrying medium. The research was made on 16 rabbits, where a 3.0 mm cylindrical osteochondrous defect of both femoral processes was modelled with receiving of an explant. The osteochondrous explant was used for culturing the autoCC articular cartilage ex vivo during 27-35 days, after that an implantation was performed. It was revealed that a large full-thickness injury of articular surfaces did not restore by itself with formation of a full-fledged hyaline cartilage, but healed according to the type of an incomplete repair, that in terms of arthrology could be determined as progressive osteoarthrosis at stages II-IV. Implantation of ex vivo cultured autoCC in the form of columns on a gel carrier into the area of pathological changes in the articular surface without any special fixing apparently did not affect significantly the dynamics of repair of the articular surface damaged, and autoCC themselves died due to necrosis, lysis or apoptosis.


Регенерация суставного хряща / Н. В. Дедух, С. В. Малышкина, В. А. Радченко и др. // Остеоартроз: консервативная терапия / под ред. Н. А. Коржа, Н. В. Дедух, И. А. Зупанца. — Харьков: Золотые страницы, 2007. — С. 87–134.

Дедух Н. В. Скелетные ткани / Н. В. Дедух, Е. Я. Панков // Руководство по гистологии. — СПб: СпецЛит, 2001. — Т. 1. — С. 284–336.

Лаврищева Г. И. Кость и хрящ / Г. И. Лаврищева, Л. Н. Михайлова // Структурные основы адаптации и компенсации нарушенных функций. Руководство / под ред. Д. С. Саркисова. — М.: Медицина, 1987. — С. 154–185.

Павлова В.Н. Репаративные потенции хряща / В. Н. Павлова, Т. Н. Копьева // Хрящ / В. Н. Павлова, Т. Н. Копьева, Л. И. Слуцкий, Г. Г. Павлов. — М.: Медицина, 1988. — С. 191–196.

Хэм А. Гистология / А. Хэм, Д. Кормак. — М.: Мир, 1983. — Т. 3. — С. 136–162.

A prospective, randomised comparison of autologous chondrocyte implantation versus mosaicplasty for osteochondral defects in the knee / G. Bentley, L. C. Biant, R. W. J. Carrington et al. // J. Bone Joint Surg. — 2003. — Vol. 85-B, № 2. — P. 223–230.

Histological evaluation of chondral defects after autologous chondrocyte implantation of the knee / T. W. R. Briggs, S. Mahroof, L. A. David et al. // J. Bone Joint Surgery. — 2003. — Vol. 85-B, № 7. — P. 1077–1083.

Articular Cartilage Engineering with Autologous Chondrocyte Transplantation: A Review of Recent Developments / M. Brittberg, L. Peterson, E. Sjogren-Jansson et al. // J. Bone Joint Surgery. — 2003. — Vol. 85-A, Suppl. 3. — P. 109–115.

Buckwalter J.A. Articular Cartilage. Part II: degeneration and osteoarthrosis, repair, regeneration, and transplantation / J. A. Buckwalter, H. J. Mankin // J. Bone Joint Surg. — 1997. —Vol. 79-A, № 4. — P. 612–632.

Autologous chondrocyte implantation. Culture in a TGF-β-containing medium enhances the reexperession of a chondrocytic phenotype in passaged human chondrocytes in pellet culture / A. J. Goldberg, D. A. Lee, D. L. Bader, G. Bentley // J. Bone Joint Surg. — 2005. — Vol. 87-B, № 1. — P. 128–134.

Molecular and immunohistological characterization of human cartilage two years following autologous cell transplantation / B. Grigolo, L. Roseti, L. de Franceschi, A. Piacentini et al. // J. Bone Joint Surg. — 2005. — Vol. 87-A, № 1. — P. 46–57.

Prospective clinical Study of autologous chondrocyte implantation and correlatoin with MRI at three and 12 months /

I. J. P. Henderson, B. Tuy, D. Connell et al. // J. Bone Joint Surgery. — 2003. — Vol. 85-B, № 7. — P. 1060–1066.

Autologous Chondrocyte Implantation and Osteochondral Cylinder Transplantation in Cartilage Repair of the Knee Joint. A Prospective, Comparative Trial / U. Horas, D. Pelinkovic, G. Herr et al. // J. Bone Joint Surg. — 2003. — Vol. 85-A, № 2. — P. 185–192.

Chondrocyte Death Associated with Human Femoral Osteochondral Harvest as Performed for Mosaicplasty / J. S. Huntley, P. G. Bush, J. M. McBirnie et al. // J. Bone Joint Surg. — 2005. — Vol. 87-A, № 2. — P. 351–360.

Hunziker E. B. Articular Cartilage Repair / E. B. Hunziker, L. Rosenberg // Arthritis and allied conditions. A textbook of rheumatology / Ed. W. J. Koopman. — 13-th ed. — Baltimore: Williams & Wilkins, 1997. — Vol. 2. — P. 2027–2038.

Autologous Chondrocyte Implantation Compared with Microfracture in the Knee. A Randomized Trial / G. Knutsen, L. Engebretsen, T. C. Ludvigsen, J. O. Drogset // J. Bone Joint Surg. — 2004. — Vol. 86-A, № 3. — P. 455–464.

Repair of defects in articular joints. Prospects for Material-Based Solutions in Tissue Engineering / A. K. Lynn, R. A. Brooks, W. Bonfield, N. Rushton // J. Bone Joint Surg. — 2004. — Vol. 86-B, № 8. — P. 1093–1099.

Autogenous Osteochodral Morselised Grafts for Full Thickness Osteochodral Defects in the Knee Joints of Pigs / A. Mahadev, D. P. Mahara, P. Chang et al. // Singapore Med. J. — 2001. — Vol. 42, № 9. — P. 410–416.

Chodrocyte transplantation into articular cartilage defects with use of calcium alginate: the fate of the cells / C. M. Mierisch, H. A. Wilson, M. A. Turner et al. // J. Bone Joint Surg. — 2003. — Vol. 85-A, № 9. — P. 1757–1767.

Tissue-engineered growth of cartilage: the effect of varying the concentration of chondrocytes seeded onto synthetic polymer matrices / W. C. Puelacher, S. W. Kim, J. P. Vacanti et al. // Int. J. Oral Maxillofacial Surg. — 1994. — Vol. 23, № 1. — P. 49–53.

Redman S. N. Current Strategies for Articular Cartilage Repair / S. N. Redman, S. F. Oldfield, C. W. Archer // European Cells and Materials. — 2005. — Vol. 9, № 1. — P. 23–32.

Articular Cartilage Repair / R. L. Sah, A. C. Chen, S. S. Chen et al. // Arthritis and allied conditions. A textbook of rheumatology / Ed. W. J. Koopman. — 14-th ed. — Philadelphia: Lippincott Williams & Wilkins, 2001. — Vol. 2. — P. 2264–2280.

Saris D. B. F. Joint Homeostasis. The discrepancy between old and fresh defects in cartilage repair / D. B. F. Saris, W. J. A. Dhert, A. J. Verbout // J. Bone Joint Surg. — 2003. — Vol. 85-B, № 7. — P. 1067–1076.

The treatment of osteochondral lesions using a combination of autologous chondrocyte implantation and autograft. Three-year follow-up / J. R. Sharpe, S. U. Ahmed, J. P. Fleetcroft, R. Martin // J. Bone Joint Surg. — 2005. — Vol. 87-B, № 5. —

P. 730–735.