Experimental and histological study of reparative osteogenesis in conditions of different methods of fixation during allograft alocompositive arthroplasty of long bones





allocomposite endoprosthetics, reparative osteogenesis, femur, rats


The study of bone regeneration in the case of application of al­locomposite endoprosthetics is an important area of research in orthopedics.

Objective: to study the regeneration processes using allocomposite endoprosthetics to replace postresection segmental defects of the femur with various fixation methods in rats.

Methods: models of various variants of fixing the al­lograft to the bone of the recipient have been developed and improved; a model of cementless fixation of an endoprosthe­sis with ceramic stem coating using an allograft has been pro­posed. The experiment was carried out on 45 laboratory white rat cells (age 5 months, body weight 350–400 g), which were divided into three groups of 15 each: I — transverse osteotomy of the femur with implantation of the allocomposite endopros­thesis; II — transverse osteotomy of the femur with implantation of the allocomposite endoprosthesis and autoplasty of junctional zone between bone and allograft; III — stepped osteotomy with implantation of the allocomposite endoprosthesis. The ani­mals were sacrificed at 3, 6 and 9 months, and histological examination was performed.

Results: after transverse osteotomy of the femur (group I and II) and substitution of postresection defects of the proximal part with allocomposite endoprostheses, allografts were reorganized at all terms of the study. Symptoms of inflammation are not established in any case. With the in­crease in the contact plane of the allograft with the recipient bone (step osteotomy, group III), the processes of reorganiza­tion and vascularization of the allograft were more pronounced. Signs of active reorganization of autografts were observed from the 3rdmonth of the experiment. Bone fusion of an autograft with a bone of the recipient is established after 9 months after operation. On the perimeter of the endoprosthesis, the newly formed bone tissue was in tight contact with the ceramic coating of the endoprosthesis.

Author Biographies

Oleg Vyrva

Sytenko Institute of Spine and Joint Pathology, Kharkiv. Ukraine

MD, Prof. in Orthopaedics and Traumatology



Yanina Golovina

Sytenko Institute of Spine and Joint Pathology, Kharkiv. Ukraine

PhD in Orthopaedics and Traumatology



Roman Malyk

Sytenko Institute of Spine and Joint Pathology, Kharkiv. Ukraine




Zinayda Danishchuk

 Sytenko Institute of Spine and Joint Pathology, Kharkiv. Ukraine




Olga Nikolchenko

 Sytenko Institute of Spine and Joint Pathology, Kharkiv. Ukraine

PhD in Biol. Sci. 





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